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Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli, High Copy Plasmid DFS Item

Ideal for high-efficiency cloning and plasmid propagation

$301.35 - $1,050.00

Specifications

Bacterial Content TOP10
Cell Type Chemically Competent
Contains One Shot TOP10 Chemically Competent E. coli: 42 vials, 50μL each, pUC19 DNA (10pg/μL): 2 vials, 50μL each, S.O.C. medium: 2 bottles, 6mL each
Contains F' Episome No
Efficiency > 1x109
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

C404010

DFS Item
 
life technologies
C404010
10 reactions Each for $301.35

C404006

DFS Item
 
life technologies
C404006
40 reactions Each for $1,050.00

C404003

DFS Item
 
invitrogen™
C404003
20 reactions Each for $564.90
Description & Specifications

Specifications

Bacterial Content TOP10
Cell Type Chemically Competent
Contains One Shot TOP10 Chemically Competent E. coli: 42 vials, 50μL each, pUC19 DNA (10pg/μL): 2 vials, 50μL each, S.O.C. medium: 2 bottles, 6mL each
Contains F' Episome No
Efficiency > 1x109
For Use With (Application) Cloning, Transformation
Improves Plasmid Quality Yes
Includes Blue/White Screening Yes
Recombinant Yes
Species E. coli
Storage Requirements Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
T1 Phage Resistant No

One Shot TOP10 Chemically Competent E. coli are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA. They allow stable replication of high-copy number plasmids and are the same competent cells that come with many Life Technologies cloning kits.

  • Maximization of cloning efficiency in a single-tube format
  • Enhanced genomic DNA cloning capabilities

Not high-throughput compatible (Manual)

Cloning, Transformation

Shipping Condition: Dry Ice