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Thermo Scientific™ DH5α Competent Cells; High Efficiency

Numéro de catalogue. FEREC0112
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20 Reactions
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Numéro de catalogue. Quantity
FEREC0112 20 Reactions
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Numéro de catalogue. FEREC0112 Fournisseur Thermo Scientific™ Code fournisseur EC0112
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Description

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

  • The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gal
  • RecA1 and endA1 mutations in DH5α cells improve insert stability and quality of the extracted plasmid DNA
  • High transformation efficiency: >1x109 cfu/μg pUC19 DNA
  • Suitable for routine and high-throughput cloning applications
  • Genetic markers that allow for blue/white colony screening
  • Convenient two reactions per vial packaging
  • S.O.C. Outgrowth medium included
  • DH5α Competent Cells are suitable for variety of applications requiring high transformation efficiency:
    • Efficient DNA cloning derived from PCR, cDNA-generation reactions
    • recA1 marker facilitates working with difficult to transform DNA
    • Ideal for generation of cDNA libraries using plasmid-derived vectors
    • Site-directed mutagenesis
  • Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1

Spécifications

Description Chemically Competent
Contains F' Episome Lacks F' Episome
Content And Storage Dry Ice (-80°C)
Bacterial or Yeast Strain DH5α
Species E. coli
Format Tube
For Use With (Application) Cloning
Quantity 20 Reactions