Gel Filtration Media and Columns

A variety of columns and media for the size-based separation and purification of proteins, lipoproteins, peptides, etc. using gel filtration chromatography techniques.

1 – 15 of 44 results

Cytiva HiLoad™ 16/600 Superose™ 6 Prep Grade Preparative SEC Column

A prepacked SEC column for high-resolution purification of large biomolecules and protein complexes with sample volumes up to 5 mL

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Cytiva Sephadex™ G50 Fine Media

Suitable for gel filtration

Pricing and Availability
Specifications

Height (Metric)	10cm
Content And Storage	4 to 30°C
Bed Volume	9 to 11mg/g
pH Range	2 to 10
Diameter (Metric) Inner	5cm
For Use With (Application)	For the separation of molecules M_r >30 000 from molecules M_r <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids
Max. Pressure	min 150 cm/h, bed height 10 cm, column 5 cm i.d.
Particle Size	34 to 208 μm in wet form, 20 to 80 μm in dry form
Flow Rate	< Darcy´s Law
Format	Solid Bead
Product Type	Sephadex G50 Media
Sample Type	DNA
Support Type	Cross-linked Dextran
Height (English)	3.93 in.

Cytiva Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

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Cytiva Sephadex™ G50 Medium Media

Suitable for gel filtration

Pricing and Availability
Specifications

Particle Size	100 to 300 μm in wet form, 50 to 150 μm in dry form
Height (Metric)	10cm
Bed Volume	9 to 11mg/g
Format	Solid Bead
pH Range	2 to 10
Product Type	Sephadex G50 Media
Sample Type	DNA
Diameter (Metric) Inner	5cm
Support Type	Cross-linked Dextran
Height (English)	3.93 in.

Cytiva Sephadex™ G-50 and G-100 DNA Grade Agarose Gels

Used for purification of DNA from small molecules. Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.

Pricing and Availability
Specifications

Content And Storage	4° to 30°C
Format	Beaded Gel
pH Range	2 to 10
Product Type	G-50 and G-100 DNA Grade Agarose Gels
For Use With (Equipment)	Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column
Sample Type	DNA
Support Type	Cross-linked Dextran with Epichlorohydrin

Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Pricing and Availability
Specifications

Particle Size	34 μm
Ligand Type	10μM NHS groups/mL medium
Height (Metric)	2.5cm
Content And Storage	4° to 8°C
pH Range	3 to 12
Product Type	NHS-Activated HP Column
For Use With (Equipment)	Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
Material	Polypropylene
For Use With (Application)	Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
Support Type	6% Highly Cross-linked Agarose
Max. Pressure	42psi
Height (English)	0.98 in.

Cytiva Sephacryl™ S-400 High Resolution Media

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

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Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns

For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.

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Cytiva HiTrap™ Capto™ Core 400

Designed for intermediate purification and polishing of viruses and other large biomolecules

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Cytiva Sephadex G-50 Medium, 500g

Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.

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Cytiva Sephadex™ G15 Media

Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight

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Cytiva Sephadex™ G75 Superfine Media

Classic gel filtration medium

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Cytiva Sephadex™ G10 Media

Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules

Pricing and Availability
Specifications

Height (Metric)	30cm
Content And Storage	4 to 30°C
Bed Volume	2 to 3mL/g in Distilled Water
pH Range	2 to 13
Diameter (Metric) Inner	2.6cm
For Use With (Application)	Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W.
Max. Pressure	24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d
Particle Size	55 to 165 μm in wet form, 40 to 120 μm in dry form
Flow Rate	< Darcy´s Law
Format	Solid Bead
Product Type	Sephadex G10 Media
Sample Type	Peptides, Small Biomolecules
Support Type	Cross-linked Dextran
Height (English)	11.81 in.

Cytiva Sephadex™ G-100 Media

Commonly used for desalting and buffer exchange of very large biomolecules

Pricing and Availability
Specifications

Content And Storage	4° to 30°C
Bed Volume	15 to 20mL/g in Distilled Water
Format	Solid Bead
pH Range	2 to 10
Product Type	Sephadex G-100 Media
Sample Type	Large biomolecules
Quantity	100 g
For Use With (Application)	Useful for desalting and Buffer exchange of very large biomolecules
Support Type	Cross-linked Dextran
Max. Pressure	0.096 bar

Cytiva Sephadex™ Ion Exchange Media

Separate molecules over broad molecular weight and pH ranges

Pricing and Availability
Specifications

Height (Metric)	5 to 15cm
Content And Storage	4° to 30°C
Format	Solid Spherical Bead
Product Type	Sephadex Ion Exchange Media
For Use With (Equipment)	Column or Batch Techniques
For Use With (Application)	Separating and purifying Proteins, Polypeptides, Nucleic Acids and other charged biomolecules by using column or batch techniques
Support Type	Cross-linked Dextran
Height (English)	2 to 5.9 in.

Gel Filtration Media and Columns

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