missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Gel Filtration Media and Columns
  6. Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Catalog No. p-4591249
Change view
Click to view available options
Flow Rate:
<0.5 mL/min.
<1.3 mL/min.
Bed Volume:
120 mL
320 mL
Diameter (Metric) Inner:
1.6cm
2.6cm
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Flow Rate Bed Volume Diameter (Metric) Inner
45002992 <1.3 mL/min. 120 mL 1.6cm
45002990 <0.5 mL/min. 120 mL 1.6cm
45002993 <0.5 mL/min. 320 mL 2.6cm
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. 45002992 Supplier Cytiva Supplier No. 28935606
Only null left

Description

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

Specifications

Quantity Each
Flow Rate <1.3 mL/min.
Particle Size 25 to 75 μm
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Bed Volume 120 mL
Product Type Sephacryl HR Pre-packed Gel Filtration Column
Diameter (Metric) Inner 1.6cm
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Height (English) 23.62 in.
Height (Metric) 60cm
Material Polypropylene
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
pH Range 3 to 11
Support Type Allyl Dextran and N,N'-methylenebisacrylamide
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Content And Storage 4°C to 30°C, 20% Ethanol
Show More Show Less