Gel Filtration Media and Columns
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Filtered Search Results
Cytiva Sephadex™ LH-20
Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.
| Particle Size | 18 to 111 μm in dry form |
|---|---|
| Height (Metric) | 30cm |
| Flow Rate | 720 cm/hr. |
| Content And Storage | 4 to 30°C |
| Format | Solid Spherical Bead |
| pH Range | 2 to 13 |
| Product Type | Sephadex LH-20 Media |
| Sample Type | Steroid, Terpenoid, Lipid, low molecular weight Peptide |
| Diameter (Metric) Inner | 2.6cm |
| For Use With (Application) | For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures |
| Support Type | Hydroxypropylated, cross-linked Dextran |
| Max. Pressure | 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d. |
| Height (English) | 11.81 in. |
| Particle Size | 25 to 75 μm |
|---|---|
| Height (Metric) | 60cm |
| Content And Storage | 4° to 30°C |
| pH Range | 3 to 11 |
| Product Type | Sephacryl HR Column |
| For Use With (Equipment) | Low to medium pressure AKTA™ systems |
| Sample Type | Antibodies, serum proteins and mid-size proteins |
| Quantity | Each |
| Material | Polypropylene |
| For Use With (Application) | Separation of biomolecules with maximum recovery and minimum nonspecific adsorption with long term chemical and physical stability resulting from the hydrophilic, rigid Allyl Dextran/Bisacrylamide matrix |
| Max. Pressure | 22psi |
| Height (English) | 23.62 in. |
Cytiva Superdex 30 Increase Precision 3.2/300 GL Prepacked SEC Column
Designed for high resolution in small-scale preparative purification and analysis for sample volumes up to 500μL
Cytiva HiTrap™ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciences™ HiTrap™ Heparin HP Columns are prepacked with Heparin Sepharose™ High Performance for high-resolution purification.
| Particle Size | 34 μm |
|---|---|
| Ligand Type | 10mg Heparin/mL medium |
| Height (Metric) | 2.5cm |
| Content And Storage | 4° to 30°C |
| pH Range | 5 to 10 |
| Product Type | Heparin HP Column |
| For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
| Material | Polypropylene |
| For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
| Support Type | 6% Highly Cross-linked Agarose |
| Max. Pressure | 42psi |
| Height (English) | 0.98 in. |
Cytiva Sephadex™ G10 Media
Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules
| Height (Metric) | 30cm |
|---|---|
| Content And Storage | 4 to 30°C |
| Bed Volume | 2 to 3mL/g in Distilled Water |
| pH Range | 2 to 13 |
| Diameter (Metric) Inner | 2.6cm |
| For Use With (Application) | Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W. |
| Max. Pressure | 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d |
| Particle Size | 55 to 165 μm in wet form, 40 to 120 μm in dry form |
| Flow Rate | < Darcy´s Law |
| Format | Solid Bead |
| Product Type | Sephadex G10 Media |
| Sample Type | Peptides, Small Biomolecules |
| Support Type | Cross-linked Dextran |
| Height (English) | 11.81 in. |
| Height (Metric) | 5 to 15cm |
|---|---|
| Content And Storage | 4° to 30°C |
| Format | Solid Spherical Bead |
| Product Type | Sephadex Ion Exchange Media |
| For Use With (Equipment) | Column or Batch Techniques |
| For Use With (Application) | Separating and purifying Proteins, Polypeptides, Nucleic Acids and other charged biomolecules by using column or batch techniques |
| Support Type | Cross-linked Dextran |
| Height (English) | 2 to 5.9 in. |
Cytiva Superdex™ 75 Prep Grade
Offers high resolution fractionation for separation, polishing and product formulation of recombinant proteins
Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns
For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids
| Particle Size | 25 to 75 μm |
|---|---|
| Height (Metric) | 60cm |
| pH Range | 3 to 11 |
| Product Type | Sephacryl HR Pre-packed Gel Filtration Column |
| For Use With (Equipment) | Low to medium pressure AKTA™ systems or pumped chromatography systems |
| Sample Type | Polysaccharides, macromolecules with extended structures and even small plasmids |
| Quantity | Each |
| Material | Polypropylene |
| For Use With (Application) | Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids |
| Support Type | Allyl Dextran and N,N'-methylenebisacrylamide |
| Max. Pressure | 1.5 bar [0.15 MPa] (22 psi) |
| Height (English) | 23.62 in. |
Cytiva Superose™ Media Prep Grade Lab Packs
High performance gel filtration media for scaleup to preparative purifications of biomolecules
| Particle Size | 30 to 40 μm |
|---|---|
| Flow Rate | 40 cm/hr. |
| Bed Volume | ≈100 mL |
| pH Range | 3 to 12 |
| For Use With (Equipment) | AKTA™ design pump or system |
| Sample Type | Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules |
| Quantity | Each |
| For Use With (Application) | Separations of Proteins and other biomolecules according to size |
| Support Type | Highly Cross-linked Agarose |
Cytiva Sephadex™ G-50 and G-100 DNA Grade Agarose Gels
Used for purification of DNA from small molecules. Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.
| Content And Storage | 4° to 30°C |
|---|---|
| Format | Beaded Gel |
| pH Range | 2 to 10 |
| Product Type | G-50 and G-100 DNA Grade Agarose Gels |
| For Use With (Equipment) | Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column |
| Sample Type | DNA |
| Support Type | Cross-linked Dextran with Epichlorohydrin |
Cytiva Superdex™ 200 Increase 3.2/300 Prepacked Precision Column
Used for micro-scale (μg) preparative purification and for characterization and analysis of small volumes (4 to 50μL) of proteins. Cytiva Lifescience™ Superdex™ 200 Increase 3.2/300 Prepacked Precision Column desigened for size exclusion chromatography.
Cytiva Sephadex™ G-25 DNA Grade SF Cleanup Gel
Tested to ensure reproducibly high recovery of DNA (10 bases in length) from small molecules by gel filtration
Cytiva Sephadex G-50 Medium, 500g
Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.