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Thermo Scientific™ Eco32I (EcoRV), HC (50 U/μL)
The Eco32I (EcoRV) restriction enzyme recognizes GAT^ATC sites and cuts best at 37°C in R buffer (Isoschizomers: EcoRV).
Thermo Scientific™ Eco47III (AfeI) (10 U/μL)
The Eco47III (AfeI) restriction enzyme recognizes AGC^GCT sites and cuts best at 37°C in O buffer (Isoschizomers: AfeI, Aor51HI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Eco47III (AfeI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer O |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ Eco105I (SnaBI) (10 U/μL)
The Eco105I (SnaBI) restriction enzyme recognizes TAC^GTA sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstSNI, SnaBI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Eco105I (SnaBI) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ HincII (HindII) (10 U/μL)
The HincII (HindII) restriction enzyme recognizes GTY^RAC sites and cuts best at 37°C in Tango buffer (Isoschizomers: HindII).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | HincII (HindII) |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ MluI (10 U/μL)
The MluI restriction enzyme recognizes A^CGCGT sites and cuts best at 37°C in R buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Mlu I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer R |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ NcoI (10 U/μL)
The NcoI restriction enzyme recognizes C^CATGG sites and cuts best at 37°C in Tango buffer (Isoschizomers: Bsp19I).
Concentration | 10 U/μL |
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Thermo Scientific™ NotI (10 U/μL)
The NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in O buffer (Isoschizomers: CciNI).
Concentration | 10 U/μL |
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Thermo Scientific™ PvuI (10 U/μL)
The PvuI restriction enzyme recognizes CGAT^CG sites and cuts best at 37°C in R buffer (Isoschizomers: BpvUI, MvrI, Ple19I).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Pvu I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer R |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ PvuII (10 U/μL)
The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.
Concentration | 10 U/μL |
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Thermo Scientific™ SduI (Bsp1286I) (10 U/μL)
Cut at GDGCH^C sites with SduI (Bsp1286I) restriction enzyme, which performs best at 37°C in its own unique buffer (Isoschizomers: Bsp1286I, MhlI).
Thermo Scientific™ XhoI (10 U/μL)
Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).
Concentration | 10 U/μL |
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Thermo Scientific™ SspI (10 U/μL)
The SspI restriction enzyme recognizes AAT^ATT sites and cuts best at 37°C in G buffer.
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Ssp I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer G |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ HinfI, HC (50 U/μL)
Thermo Scientificâ„¢ Fermentas HinfI is available as a FastDigest enzyme which is used for rapid DNA digestion.
Thermo Scientific™ AluI (10 U/μL)
Cut at AG^CT sites with AluI restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AluBI).
Sensitive to Heat Inactivation | Yes |
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Product Type | Restriction Enzyme |
Enzyme | Alu I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentration | 10 U/μL |
Thermo Scientific™ Alw21I (BsiHKAI) (10 U/μL)
Cut at GWGCW^C sites with Alw21I (BsiHKAI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: Bbv12I, BsiHKAI).