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Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Cut at C^TYRAG sites with SmoI (SmlI) restriction enzyme, which performs best at 55°C in Tango buffer (Isoschizomers: SmlI).
Stabilize enzymes, increase PCR yields, and prevent adhesion of enzymes with Thermo Scientific™ Bovine Serum Albumin.
The FastDigest EcoRI restriction enzyme recognizes G^AATTC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Température de réaction optimale | 37°C |
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Enzymes | EcoR I |
Catégorie de recherche | Traditional Cloning |
Sensibilité à la méthylation | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Type de produit | Restriction Enzyme |
Gamme de produits | FastDigest™ |
En surplomb | 5' protruding ends |
Enzymes de restriction de type IIS | No |
Tampon compatible | 10X FastDigest Buffer/FastDigest Green Buffer |
Inactivation Temperature | 80°C |
Sensible à l’inactivation thermique | Yes |
Optimize electrophoresis of both genomic and large supercoiled DNA in agarose and polyacrylamide gels with Thermo Scientific™ 50X TAE Buffer (Tris-acetate-EDTA).
The FastDigest HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Température de réaction optimale | 37°C |
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Enzymes | Hind III |
Catégorie de recherche | Traditional Cloning |
Sensibilité à la méthylation | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Type de produit | Restriction Enzyme |
Gamme de produits | FastDigest™ |
En surplomb | 5' protruding ends |
Enzymes de restriction de type IIS | No |
Tampon compatible | 10X FastDigest Buffer/FastDigest Green Buffer |
Inactivation Temperature | 80°C |
Sensible à l’inactivation thermique | Yes |
Cut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer.
Température de réaction optimale | 37°C |
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Enzymes | BamH I |
Catégorie de recherche | Traditional Cloning |
Sensibilité à la méthylation | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Type de produit | Restriction Enzyme |
Gamme de produits | FastDigest™ |
En surplomb | 5' protruding ends |
Enzymes de restriction de type IIS | No |
Tampon compatible | 10X FastDigest Buffer/FastDigest Green Buffer |
Inactivation Temperature | 80°C |
Sensible à l’inactivation thermique | Yes |
Perform single-, double- or multiple DNA digestion within 5-15 minutes with this advanced line of restriction enzymes, 100% active in one universal buffer.
Simplify traditional restriction enzyme cloning with the Invitrogen™ Anza™ Restriction Enzyme Cloning System.
The FastDigest PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BspMAI).
Température de réaction optimale | 37°C |
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Enzymes | Pst I |
Catégorie de recherche | Traditional Cloning |
Sensibilité à la méthylation | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Type de produit | Restriction Enzyme |
Gamme de produits | FastDigest™ |
En surplomb | 3' protruding ends |
Enzymes de restriction de type IIS | No |
Tampon compatible | 10X FastDigest Buffer/FastDigest Green Buffer |
Sensible à l’inactivation thermique | No |
The HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer
Thermo Scientific ATP (adenosine 5'-triphosphate) is an extremely stable nucleotide, and it is supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.
Tampon de stockage | 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol |
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Composants | 25 to 50% Water, >50% Glycerin |
Type de produit | NotI |
Site de coupe | GC.GGCCGC |
pH | 7.4 |
Concentration | 10 U/μL |
Forme | Liquid |
Contenu et stockage | Keep container tightly closed |
À utiliser avec (application) | >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I |
Tampon de stockage | 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol |
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Température de l’incubateur | 37°C |
Composants | 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride |
Type de produit | BglII |
Site de coupe | A.GATCT |
pH | 7.4 |
Concentration | 10 U/μL |
Forme | Liquid |
Contenu et stockage | Keep container tightly closed |
À utiliser avec (application) | >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII |