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ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1

Invitrogen™ ELISA Stop Solution  DFS Item

A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.

Shipping Condition Ambient
Content And Storage Room Temperature
Form Liquid
Product Type ELISA Stop Solution
Substrate Type HRP, TMB
2

Invitrogen™ eBioscience™ TMB Solution (1X)

Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.

3

Invitrogen™ eBioscience™ ELISA/ELISPOT Diluent (5X)

Supplemented with fetal bovine serum as source of animal serum proteins. Used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples and making the working dilutions of secondary detection reagent. Also contains 1% ProClin 150 as a preservative

4

Invitrogen™ DynaLight™ Substrate with RapidGlow™ Enhancer  DFS Item

A ready-to-use chemiluminescent substrate formulation optimized to achieve faster results in solution-based immunoassays. Thermo Scientific DynaLight Substrate with RapidGlow Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline

Content And Storage 1 amber bottle — Store at 2 to 8°C
Substrate Properties Chemical Substrate
Format Bottle
For Use With (Equipment) Luminometer (Microplate)
Form Liquid
Product Type ELISA Substrate
Sample Type Cell Cultures, Whole Blood, Serum
Substrate Type Alkaline Phosphatase
Assay Sensitivity 1 pg/mL
High-throughput Compatibility High-throughput Compatible
Test Time 2 min.
Product Line DynaLight, NovaBright, RapidGlow
Detection Method Chemiluminescence
5

Invitrogen™ Luminescence Enhancer For Solution Assays, Sapphire-II™ Enhancer  DFS Item

Chemiluminescence enhancer that significantly reduces aqueous quenching and tune the wavelength of light emission.

6

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

7

Invitrogen™ ELISA-LIGHT W/CSPD/EMERALD II  DFS Item

Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

8

Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

9

Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer  DFS Item

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

10

Invitrogen™ Wash Buffer (25X)  DFS Item

Suitable for use with all ELISA assays

Shipping Condition Wet Ice
11

Invitrogen™ ELISA-LIGHT W/CDP-STAR/EII EAC  DFS Item

Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

12

Thermo Scientific™ QuantaBlu™ NS/K Fluorogenic Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

13

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
14

Invitrogen™ ELISA-LIGHT CSPD/SAPPHIRE-II E  DFS Item

Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.

15

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence