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Thermo Scientific™ Pierce™ ABTS Substrates


Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Manufacturer: thermo scientific™  37615

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Catalog No. PI37615

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Description & Specifications

Specifications

Description Pierce 1-Step ABTS Solution
Formulation Proprietary buffered solution containing 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt
Quantity 250mL
Sufficient For At least 15 x 96-well microplates using 150uL per well

Thermo Scientific™ Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods.

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower (approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Highlights:

  • Excellent choice when maximum sensitivities are not required
  • Less readily oxidized and color development is slower (approximately 20 minutes) than OPD and TMB