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Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Catalog No. PI37615
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250 mL
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PI37615 250 mL
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Catalog No. PI37615 Supplier Thermo Scientific™ Supplier No. 37615
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Description

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Thermo Scientific™ Pierce ABTS Substrates are tablets and a ready-to-use solution of a water-soluble peroxidase substrate that yields a measurable green end product for use in ELISA methods.

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower (approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Highlights:

  • Excellent choice when maximum sensitivities are not required
  • Less readily oxidized and color development is slower (approximately 20 minutes) than OPD and TMB

Specifications

Description Pierce 1-Step ABTS Solution
Product Type ELISA Substrate
Quantity 250 mL
Detection Method Colorimetric
Product Line 1-Step
Substrate Properties Chemical Substrate
Substrate Type HRP (Horseradish Peroxidase)
Content And Storage Store at 4°C

For Research Use Only. Not for use in diagnostic procedures.