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Invitrogen™ ELISA Buffer Kit

Catalog No. CNB0011
Encompass
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Quantity:
10 plates
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Catalog No. Quantity
CNB0011 10 plates
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Catalog No. CNB0011 Supplier Invitrogen™ Supplier No. CNB0011

Description

Includes

Coating Buffer A (100mL): 1 bottle, Coating Buffer B (100mL): 1 bottle, Assay Buffer (5x) (200mL): 1 bottle, Wash Buffer (25x) (100mL): 3 bottles, Stabilized Chromogen (25mL): 4 bottles, Stop Solution (100mL): 1 bottle.

Concentrated

Buffer Kit for Antibody Pairs is for use with Invitrogen Antibody Pairs. Kit provides sufficient materials for ten ELISA plates.

Build Your Own Immunoassay, Cell Analysis, Immunoassays

Order Info

Shipping Condition: Wet Ice

Specifications

Quantity 10 plates
Form Concentrate
For Use With (Application) ELISA
Content And Storage Coating Buffer A (100 mL): 1 bottle
Coating Buffer B (100 mL): 1 bottle
Assay Buffer (5x) (200 mL): 1 bottle
Wash Buffer (25x) (100 mL): 3 bottles
Stabilized Chromogen (25 mL): 4 bottles
Stop Solution (100 mL): 1 bottle. Store at 2°C to 8°C.
Shipping Condition Wet Ice

Frequently Asked Questions (FAQs)

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

For Research Use Only. Not for use in diagnostic procedures.