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Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)

Catalog No. 50246701
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100 mL
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50246701 100 mL
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Catalog No. 50246701 Supplier Invitrogen™ Supplier No. IOCLB1
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Description

Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.

  • The 5x Cell Lysis Mix is used to lyse cells in the presence of culture medium and is typically used to lyse non-adherent cells
  • Dilute 5x Cell Lysis Mix 5-fold with water to make a 1x Cell Lysis Mix
  • 1x Mix is used to lyse cells after the removal of culture medium, and is typically used to lyse adherent cells or non-adherent cells that have been harvested by centrifugation
  • Lysis Mix should be used as the diluent for any dilution of cellular lysates that are required
  • Supplementing Cell Lysis Mix with extra components (e.g. protease inhibitors, chelating agents, detergents) should be tested on a case-by-case basis for compatibility with InstantOne ELISA assays
  • Included in the InstantOne ELISA kits

Specifications

Product Type ELISA Assay/Lysis Buffer
Quantity 100 mL
Form Liquid
For Use With (Application) ELISA

For Research Use Only. Not for use in diagnostic procedures.