Invitrogen™ eBioscience™ TMB Solution (1X)
Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.
Manufacturer: Invitrogen™ 00420156
TMB (3,3’,5,5’-Tetramethylbenzidine) is a substrate for horseradish peroxidase (HRP). During the enzymatic reaction the TMB substrate will result in a blue color that can be read on a spectrophotometer at 650nm. This kinetic reaction can be stopped using 1M phosphoric acid resulting in a color change to yellow that can be read at 450nm.
|HRP (Horseradish Peroxidase) Substrate|
For Research Use Only