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Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Catalog No. PI34055
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PI34055 5 g
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Catalog No. PI34055 Supplier Thermo Scientific™ Supplier No. 34055
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Description

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

Thermo Scientific™ Pierce ONPG Substrate is the preferred colorimetric substrate for ELISA applications involving β-galactosidase (β-Gal) as the reporter enzyme.

ONPG (ω-nitrophenyl-β-D-galactopyranoside) is the preferred colorimetric substrate for ELISA applications involving β-galactosidase (β-Gal) reporter enzyme. The enzyme-substrate reaction yields a yellow product with an absorbance maximum at 420nm but whose intensity is also very high at 405nm (matching a common plate reader filter set).

Highlights:

  • Product formed is completely soluble
  • High extinction coefficient at 405nm
  • Substrate yields a yellow product that is easily detectable in the visual range after stopping the reaction with 1M sodium carbonate

Specifications

Product Type ELISA Substrate
Quantity 5 g
Detection Method Colorimetric
Substrate Properties Chemical Substrate
Substrate Type Beta-Gal
Content And Storage Upon receipt store at -20°C protected from moisture and light. Product is shipped at ambient temperature.

For Research Use Only. Not for use in diagnostic procedures.