ELISA Substrates and Reagents
Invitrogen™ ELISA Buffer Kit
Concentrated
Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
Invitrogen™ ELISA/ELISPOT Coating Buffer Powder
Designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures described in the corresponding protocols.
Invitrogen™ eBioscience™ ELISA Wash Buffer, 1L packets
Suitable for washing ELISA plate microwells. eBioscience™ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.
Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)
Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.
Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use)
This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.
Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Invitrogen™ Luminescence Enhancer For Solution Assays, Emerald-II™ Enhancer
The Emerald-II chemiluminescence enhancer significantly reduces aqueous quenching and tunes the wavelength of light emission.
Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit
Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).
Invitrogen™ Galacton-Star™ Substrate (50X Concentrate)
Concentrated chemiluminescent substrate for E.coli β-galactosidase assays. Thermo Scientific Galacton-Star Substrate (50X Concentrate) is highly sensitive which makes it ideal for detection of weak expression and for transfection normalization with o
Invitrogen™ cAMP-Screen™ Assay/Lysis Buffer
For use with the cAMP-Screen or cAmp-Screen Direct™ Immunoassay System.
Invitrogen™ Galacto-Light™ Reaction Buffer Diluent with Galacton-Plus™ Substrate
Established system with extended light emission and high sensitivity for reporter gene assays. Thermo Scientific Galacto-Light Reaction Buffer Diluent and the Galacton-Plus chemiluminescent substrate solution are used with the Galacto-Light Plus Reporter
Thermo Scientific™ ELISA Blocker Blocking Buffer
Increases signal-to-noise ratio and minimizes background
Invitrogen™ cAMP-Screen™ Wash Buffer
For use with the cAMP-Screen or cAMP-Screen Direct™ Immunoassay system.