Buffers and Standards
Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0
Filtered Search Results
| Boiling Point | 100°C |
|---|---|
| Color | Undesignated |
| Traceability to NIST | Traceable to NIST. |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution, pH 6.00 |
| Grade | Certified |
| Identification | Pass Test |
| ChemAlert Storage Symbol | Gray |
| CAS | 1310-73-2 |
| Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Handle in accordance with good industrial hygiene and safety practice. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. . . NFPA Health:1 Flammability:0 Instability:0 |
| MDL Number | MFCD00134130 |
| Health Hazard 2 | CAUTION! |
| pH | 6.00 |
| Melting Point | 0°C |
Buffer Solution, pH 7.40 (Certified), Fisher Chemical™
Potassium Phosphate Monobasic/Sodium Hydroxide
| Boiling Point | 100°C |
|---|---|
| CAS | 7732-18-5 |
| MDL Number | MFCD00134130 |
| Traceability to NIST | Traceable to NIST. |
| Packaging | Poly Bottle |
| Physical Form | Liquid |
| pH | 7.4 |
| Chemical Name or Material | Buffer Solution, pH 7.40 |
| Grade | Certified |
| Identification | Pass Test |
| ChemAlert Storage Symbol | Gray |
| Density | 1.0g/cm³ |
| Melting Point | 0°C |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
| Name Note | 50X Solution |
|---|---|
| Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
| CAS | 6850-28-8 |
| Protease | Protease free |
| Physical Form | Liquid |
| Grade | Electrophoresis |
| Synonym | TAE |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| DNase | DNase free |
| Recommended Storage | RT |
| Filtered Through | Filtered through a 0.2-micron filter. |
| ChemAlert Storage Symbol | Gray |
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
| Molecular Weight (g/mol) | 238.30 |
|---|---|
| ChEBI | CHEBI:42334 |
| Color | White |
| Chemical Name or Material | HEPES |
| Grade | Molecular Biology |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| Identification | Pass Test |
| DNase | DNase free |
| Merck Index | 15, 4689 |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| ChemAlert Storage Symbol | Gray |
| Assay Percent Range | ≥99 % |
| PubChem CID | 23831 |
| Absorbance | 0.01 max. (0.1M solution) at 280nm |
| Percent Purity | ≥99% |
| CAS | 7365-45-9 |
| Protease | Protease free |
| Health Hazard 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
| MDL Number | MFCD00006158 |
| Health Hazard 2 | WARNING! |
| Solubility Information | Soluble in water |
| pH | 5.0 to 6.5 |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
| Molecular Formula | C8H18N2O4S |
Buffer Solution, pH 7.00 (Certified), Fisher Chemical™
Potassium Phosphate Monobasic/Sodium Hydroxide
| Color | Colorless |
|---|---|
| Traceability to NIST | Traceable to NIST. |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution, pH 7.00 |
| Grade | Certified |
| Identification | Pass Test |
| ChemAlert Storage Symbol | Gray |
| Density | 1.003g/cm³ |
| Vapor Pressure | 14mmHg at 20°C |
| CAS | 7732-18-5 |
| MDL Number | MFCD00134130 |
| pH | 7.00 |
| Synonym | Chlorine Titrimeter Reagent |
| Melting Point | 0°C |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
| CAS | 10043-35-3 |
|---|---|
| Synonym | TBE |
Buffer Solution, pH 4.00, Color-Coded Red (Certified), Fisher Chemical™
pH/temperature conversions given on label
| Boiling Point | 100°C |
|---|---|
| Description | pH/temperature conversions given on label. |
| Color | Red |
| Traceability to NIST | Traceable to NIST. |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution, pH 4.00, Color-Coded Red |
| Grade | Certified |
| Identification | Pass Test |
| ChemAlert Storage Symbol | Gray |
| Density | 1.0g/cm³ |
| Absorbance | Pass Test |
| Concentration or Composition (by Analyte or Components) | Potassium Hydrogen Phthalate |
| CAS | 16423-68-0 |
| Health Hazard 3 | Emergency Overview Contains a known or suspected carcinogen. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| MDL Number | MFCD00146206 |
| Health Hazard 2 | CAUTION! |
| Solubility Information | Soluble in water |
| pH | 4.0 |
| Specific Gravity | 1 |
| Melting Point | 0°C |
| Boiling Point | 100°C |
|---|---|
| Description | Potassium Phosphate Monobasic/Sodium Hydroxide. pH/temperature conversions given on label. |
| Color | Yellow |
| Traceability to NIST | Traceable to NIST. |
| Physical Form | Liquid |
| Chemical Name or Material | Buffer Solution, pH 7.00, Color-Coded Yellow |
| Grade | Certified |
| Identification | Pass Test |
| ChemAlert Storage Symbol | Gray |
| Density | 1.0g/cm³ |
| Vapor Pressure | 760mmHg at 20°C |
| Absorbance | Pass Test |
| CAS | 1934-21-0 |
| Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Handle in accordance with good industrial hygiene and safety practice. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Obtain medical attention. . . NFPA Health:1 Flammability:0 Instability:0 |
| MDL Number | MFCD00134130 |
| Health Hazard 2 | CAUTION! |
| Solubility Information | Soluble in water |
| pH | 7.00 |
| Specific Gravity | 1 |
| Melting Point | 0°C |
| CAS | 67-56-1 |
|---|---|
| Health Hazard 3 | Emergency Overview Contains a known or suspected carcinogen. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| MDL Number | MFCD00146206 |
| Health Hazard 2 | CAUTION! |
| Color | Undesignated |
| Traceability to NIST | Traceable to NIST. |
| Physical Form | Liquid |
| pH | 4.0 |
| Chemical Name or Material | Buffer Solution, pH 4.00 |
| Grade | Certified |
| Identification | Pass Test |
| ChemAlert Storage Symbol | Gray |
| Density | 1.0054g/cm³ |
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Sodium Thiosulfate Pentahydrate Solution (0.025N (N/40)/Certified), Fisher Chemical
CAS: 10102-17-7 Molecular Formula: H10Na2O8S2 Molecular Weight (g/mol): 248.172 MDL Number: MFCD00003499 InChI Key: PODWXQQNRWNDGD-UHFFFAOYSA-L Synonym: sodium thiosulfate pentahydrate,ametox,sodium thiosulfate, pentahydrate,antichlor,tinver,disodium thiosulfate pentahydrate,unii-hx1032v43m,ccris 3952,thiosulfuric acid, disodium salt, pentahydrate,sodium hyposulfite pentahydrate PubChem CID: 61475 ChEBI: CHEBI:32150 IUPAC Name: disodium;dioxido-oxo-sulfanylidene-$l^{6}-sulfane;pentahydrate SMILES: O.O.O.O.O.[O-]S(=O)(=S)[O-].[Na+].[Na+]
| PubChem CID | 61475 |
|---|---|
| CAS | 10102-17-7 |
| Molecular Weight (g/mol) | 248.172 |
| ChEBI | CHEBI:32150 |
| MDL Number | MFCD00003499 |
| SMILES | O.O.O.O.O.[O-]S(=O)(=S)[O-].[Na+].[Na+] |
| Synonym | sodium thiosulfate pentahydrate,ametox,sodium thiosulfate, pentahydrate,antichlor,tinver,disodium thiosulfate pentahydrate,unii-hx1032v43m,ccris 3952,thiosulfuric acid, disodium salt, pentahydrate,sodium hyposulfite pentahydrate |
| IUPAC Name | disodium;dioxido-oxo-sulfanylidene-$l^{6}-sulfane;pentahydrate |
| InChI Key | PODWXQQNRWNDGD-UHFFFAOYSA-L |
| Molecular Formula | H10Na2O8S2 |
Thermo Scientific™ Orion™ pH Buffer Bottles
Perform accurate, reproducible pH calibrations with these color-coded buffer bottles.
| For Use With (Equipment) | pH Meter and Electrode |
|---|---|
| Traceability to NIST | Yes |
| Physical Form | Liquid |
| Chemical Name or Material | pH Buffer, bottles |
Thermo Scientific™ Orion™ Conductivity Calibration Standards
High-accuracy conductivity calibration standards traceable to NIST™ standard reference materials, designed for reliable and reproducible measurements in lab and field. Available in a wide range of conductivity values to support consistent calibration, reliable measurements and regulatory.
| Boiling Point | 100°C |
|---|---|
| Type | Conductivity Calibration Standard |
| Description | Conductivity Standard |
| Solubility | Soluble |
| Color | Clear |
| Traceability to NIST | Yes |
| Solution Type | Standard Solution |
| Name Note | Fehling Solution A |
|---|---|
| CAS | 7732-18-5 |
| Color | Blue |
| Physical Form | Liquid |
| pH | ∼4 |
| Chemical Name or Material | Fehling's Copper Solution |
| Grade | Laboratory |