Protein Labeling Reagents
Thermo Scientific™ Alexa Fluor™ 420 NHS Ester (Succinimidyl Ester)
Alexa Fluor 420 NHS Ester is an amine-reactive dye optimized for multiplex IHC and spatial biology. A long Stokes shift and 420/500 ex/em make it a good match for the CFP filter.
Molecular Probes™ Alexa Fluor™ 488 5-SDP Ester (Alexa Fluor™ 488 Sulfodichlorophenol Ester)
Alexa Fluor™ 488 5-SDP ester (Alexa Fluor™ 488 sulfodichlorophenol ester)
Molecular Probes™ Biotin Alkyne (PEG4 carboxamide-Propargyl Biotin)
biotin alkyne (PEG4 carboxamide-propargyl biotin)
Molecular Probes™ Biotin Azide (PEG4 carboxamide-6-Azidohexanyl Biotin)
biotin azide (PEG4 carboxamide-6-azidohexanyl biotin)
Invitrogen™ Fluorescein biotin, 5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl)fluorescein
5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl)thioureidyl)fluorescein (fluorescein biotin)
Invitrogen™ BODIPY™ FL Maleimide (BODIPY™ FL N-(2-Aminoethyl))Maleimide)
BODIPY™ FL Maleimide (BODIPY™ FL N-(2-Aminoethyl))Maleimide)
Invitrogen™ Biotin-4-Fluorescein
Biotin-4-fluorescein can be used to quantitate available biotin-binding sites or the concentration of avidin or streptavidin through the strong quenching associated when this molecule binds to avidin.
Invitrogen™ BODIPY™ 650/665-X NHS Ester (Succinimidyl Ester)
BODIPY™ 650/665-X dye is bright, far-red fluorescent dye with similar excitation and emission to Cy5 or Alexa Fluor™ 647.
Thermo Scientific™ EZ-Link™ NHS-Biotin
Biotinylate protein amines (e.g., lysines) with a simple, short (13.5A), NHS-ester biotinylation reagent containing a membrane-permeable spacer arm for intracellular labeling.
Thermo Scientific™ EZ-Link™ TFPA-PEG3-Biotin
Biotinylate molecules without common functional groups for coupling with this efficient, light-activated biotinylation reagent based on tetrafluorophenyl azide.
Thermo Scientific™ EZ-Link™ Iodoacetyl-PEG2-Biotin
Biotinylate reduced cysteines (-SH groups) at high pH with this haloacetyl-activated, sulfhydryl-reactive biotinylation reagent containing polyethylene glycol for solubility.
Thermo Scientific™ EZ-Link™ Amine-PEG3-Biotin
Biotinylate proteins or surfaces using EDC or other amine-reactive crosslinkers with a mid-length (22.9A) biotinylation reagent containing a 3-unit polyethylene glycol spacer.
Thermo Scientific™ EZ-Link™ Hydrazide-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with this simplest and shortest (15.7A) hydrazide-activated biotinylation reagent.
Thermo Scientific™ EZ-Link™ Hydrazide-PEG4-Biotin
Biotinylate glycoproteins (aldehydes of oxidized sugars) with this mid-length (31.3A), pegylated biotinylation reagent containing a 4-unit PEG spacer for solubility.