missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Crosslinking, Labeling and Protein Modification
  5. Protein Labeling Reagents
  6. PI21303

Thermo Scientific™ EZ-Link™ TFPA-PEG3-Biotin

Catalog No. PI21303
Change view
Click to view available options
Quantity:
25 mg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
PI21303 25 mg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. PI21303 Supplier Thermo Scientific™ Supplier No. LSG21303
Only null left

Description

Biotinylate molecules without common functional groups for coupling with this efficient, light-activated biotinylation reagent based on tetrafluorophenyl azide.

Thermo Scientific™ EZ-Link TFPA-PEG3-Biotin is an efficient, photoactivatable reagent based on tetrafluorophenyl azide for biotinylation, and which includes a 3-unit polyethylene glycol (PEG) spacer arm.

Highlights:

  • Ideal photo-reactive biotin-labeling agent containing the efficient tetraflurophenyl azide (TFPA) moiety
  • Fluorinated aryl azide inserts into active carbon-hydrogen bonds (C-H) or unsaturated carbon chains with greater efficiency than non-fluorinated aryl azides
  • Hydrophilic polyethylene glycol (PEG) spacer contributes to improved solubility of the labeled protein
  • Flexible, non-immunogenic PEG spacer imparts a reduced tendency of the labeled protein or peptide to aggregate in solution
  • Photoactivation is achieved with a UV lamp that irradiates in the range of 300-370nm (320nm optimum)

Specifications

Product Type TFPA-PEG3-Biotin
Quantity 25 mg
Content And Storage Store desiccated at 4°C. Shipped at ambient temperature.
Product Line EZ-Link
Cell Permeability Cell-Permeant
Chemical Reactivity Light-activated
Reactive Moiety Aryl Azide
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label or Dye Biotin
Spacer Extra-long, Pegylated
Label Type Biotin & Analogs
Show More Show Less

Frequently Asked Questions (FAQs)

EZ-Link TFPA-PEG3-Biotin (Cat. No. 21303) specifies to use within a pH range of 7-8. Unfortunately, the protein we are working with requires an acidic pH for stability and rapidly aggregates in neutral or basic pH. Can the photoactivation reaction be run at lower pH?

EZ-Link TFPA-PEG3-Biotin (Cat. No. 21303) can be used in a wide variety of buffers. However, acidic pH and reducing conditions inactivate the aryl azide. We are unaware of any other photoreactive biotinylation reagents that could be used at low pH.

For Research Use Only. Not for use in diagnostic procedures.