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Thermo Scientific™ EZ-Link™ NHS-Biotin

Catalog No. PI20217
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100 mg
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Catalog No. Quantity
PI20217 100 mg
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Catalog No. PI20217 Supplier Thermo Scientific™ Supplier No. 20217
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Description

Biotinylate protein amines (e.g., lysines) with a simple, short (13.5A), NHS-ester biotinylation reagent containing a membrane-permeable spacer arm for intracellular labeling.

Thermo Scientific™ EZ-Link NHS-Biotin is the simplest NHS-ester activated biotinylation reagent for labeling primary amines (e.g., protein lysines), whose membrane permeability enables it to be used for general intracellular labeling.

Highlights:

  • Forms stable amide bonds
  • Reacts with primary amines at pH 7-9
  • Must be dissolved in DMSO or DMF and can then be transferred to an aqueous reaction solution
  • Contains a shorter spacer arm than NHS-LC-Biotin
  • Penetrates cell membranes because it is uncharged

Specifications

Product Type NHS-Biotin
Quantity 100 mg
Content And Storage Store desiccated at room temperature.
Product Line EZ-Link
Cell Permeability Cell-Permeant
Chemical Reactivity Amine
Reactive Moiety Active Ester, Succinimidyl Ester, NHS Ester
Solubility DMF (Dimethylformamide), DMSO (Dimethylsulfoxide)
Label or Dye Biotin
Spacer Short
Label Type Biotin & Analogs
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for EZ-Link NHS-Biotin?

EZ-Link NHS-Biotin is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (room temperature). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

For Research Use Only. Not for use in diagnostic procedures.