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Molecular Probes™ Alexa Fluor™ 488 5-SDP Ester (Alexa Fluor™ 488 Sulfodichlorophenol Ester)

Catalog No. A30052
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A30052 1 mg
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Catalog No. A30052 Supplier Molecular Probes™ Supplier No. A30052
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Description

Alexa Fluor™ 488 5-SDP ester (Alexa Fluor™ 488 sulfodichlorophenol ester)

The SDP (sulfodicholorphenol) ester is currently the most hydrolytically stable amine-reactive moiety available from Invitrogen. Conjugates produced with the Alexa Fluor™ 488 5-SDP ester produce the same strong amide bond between the dye and the compound of interest as succinimidyl (SE or NHS) and tetrafluorophenyl (TFP) esters, but with the improved stability in water and buffers, the SDP ester can potentially offer increased control and consistency in these reactions compared to the NHS and TFP esters.

Specifications

Quantity 1 mg
Product Type Sulfodichlorophenol Ester
Content And Storage Store in freezer (-5 to -30°C) and protect from light.
Chemical Reactivity Amine
Shipping Condition Room Temperature
Reactive Moiety Active Ester, SDP Ester
Label or Dye Alexa Fluor™ 488
Label Type Alexa Fluor
Product Line Alexa Fluor

Frequently Asked Questions (FAQs)

I am labeling a protein with Alexa Fluor 488 SDP ester. The manual recommends using a sodium bicarbonate buffer at pH 8.3. Can I use a different buffer instead?

Yes. The important thing is to use a buffered solution with a pH between 8.0 and 8.5. Do not use Tris buffer, which has amine groups. Most other buffers will work fine in that pH range. This is also true for other amine-reactive dyes, such as succinimidyl (NHS) esters or TFP esters.
I am not going to use all of my Alexa Fluor succinimidyl ester reactive dye. Can I just make it up in DMSO and store aliquots at -20 degrees C?

This is not recommended. Any trace amounts of water in the DMSO can promote spontaneous hydrolysis over time. Even if using anhydrous DMSO, DMSO is hygroscopic; it readily absorbs moisture from the atmosphere over time. A better alternative is to dissolve the reactive dye in a volatile solvent, make smaller aliquots and then evaporate off the solvent using a vacuum pump. The smaller aliquots of solid reactive dye should then be stored frozen, desiccated and protected from light. Contact Technical Support by sending an email to techsupport@thermofisher.com for the recommended volatile solvent.

For Research Use Only. Not for use in diagnostic procedures.