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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research.
Assay | BCA Assay |
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For Use With (Equipment) | Spectrophotometer, Microplate Reader |
Product Type | Protein Quantitation Assay |
For Use With (Application) | Solution-based Detection, Absorbance |
Product Line | Pierceâ„¢ |
Detection Method | Colorimetric |
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
High-quality, calibrated BSA solutions (2 mg/mL) to serially dilute to create protein assay standard curves to accurately measure protein concentration.
For isolation of RNA from cell extracts | CAS: 50-01-1 | CH6ClN3
CAS | 50-01-1 |
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Color | White |
Purity | ≥99.5% |
Physical Form | Solid |
Chemical Name or Material | Guanidine Hydrochloride |
Recommended Storage | Store at Room Temperature |
Synonym | guanidine hydrochloride |
Merck Index | 15, 4597 |
Molecular Formula | CH6ClN3 |
Formula Weight | 95.53 |
Melting Point | 178°C |
Assay Percent Range | ≥99.5% |
Perform high-throughput protein recovery (7K MWCO) and sample desalting for mass spectrometry, HPLC, capillary electrophoresis, metabolite screening, and assay development with ready-to-use Thermo Scientific Pierce Zeba desalting chromatography columns, plates, and cartridges.
MWCO | 7.0 kDa |
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Column Type | Size-exclusion, Proprietary Resin |
Purification Target | Buffer Exchange, Protein |
Product Line | Zebaâ„¢ |
Specially formulated for optimal lysis of erythrocytes in single-cell suspensions of peripheral blood and hematopoietic tissues such as spleen. This buffer can be used for lysis of human, mouse, rat, canine, and non-human primate samples.
The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.
Clear-to-purple (A750nm), 2-component, folin phenol- and copper-based reagent system to measure total protein concentration vs. protein standard.
Thermo Scientificâ„¢ EZ-Linkâ„¢ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Cell Permeability | Cell-Impermeant |
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Product Type | Sulfo-NHS-LC-Biotin |
Spacer | Mid-length |
Label or Dye | Biotin |
Solubility | DMF (Dimethylformamide),DMSO (Dimethylsulfoxide),Water |
Chemical Reactivity | Amine |
Product Line | EZ-Linkâ„¢ |
Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.
Shipping Condition | Wet Ice |
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Assay | End-point Chromogenic Assay |
Type | Endotoxin Quantitation Kit |
Target | Endotoxins |
Content And Storage | • Lyophilized amebocyte lysate • E.coli endotoxin standard • Chromogenic substrate • Endotoxin-free water Store at 2–8°C upon receipt. |
Format | Kit |
Assay Sensitivity | 0.01 EU/mL |
Assay Range | 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL |
Product Line | Pierceâ„¢ |
Detection Method | Colorimetric |
Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.
Lyse cultured mammalian cells with this high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent.
Content And Storage | Upon receipt store at 4°C. |
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Format | Liquid |
Product Type | Extraction Buffer |
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Shipping Condition | Wet Ice |
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Content And Storage | Upon receipt store desiccated at -20°C. |
Description | EDC |
Cleavable | No |
Water Soluble | Yes |
Form | Powder |
Molecular Weight (g/mol) | 191.7 |
Solubility | Water |
Labeling Method | Chemical Labeling |
Crosslinker Type | Heterobifunctional |
Chemical Reactivity | Amine-Carboxyl |
Cell Permeability | No |
Format | Standard, Single-use, Premium-grade |
Product Type | Crosslinker |
Spacer | Short (<10 AÌŠ) |
Reactive Moiety | Carbodiimide |
Spacer Arm Length | 0.0 AÌŠ |
PEGylated | No |
The new and enhanced Zeba Spin and Micro Spin Desalting columns and plates are designed for efficient desalting and buffer exchanging proteins with a molecular weight >40 kDa and removing small molecules less than 2 kDa. They provide exceptional desalting and protein recovery performance.
Disposable | Single-use |
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MWCO | 40 kDa |
Column Type | Size-exclusion, Proprietary Resin |
Purification Target | Buffer Exchange, Protein |
Product Line | Zebaâ„¢ |
Remove salts and contaminants, and maximize sample recovery using this semi-permeable membrane with a 3,500-Dalton protein MW cut-off (MWCO) cassette that is easier to use than traditional dialysis tubing.
MWCO | 3.5 kDa |
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Content And Storage | Store at room temperature |
Format | Cassette |
Membrane | Cellulose Membrane, Dialysis |
Purification Target | Buffer Exchange, Protein |
Product Line | Slide-A-Lyzerâ„¢ |