Crosslinking, Labeling and Protein Modification
Crosslinking, Labeling and Protein Modification
Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin
Thermo Scientific™ EZ-Link™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Thermo Scientific™ Pierce™ EDC, No-Weigh™ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientific™ Pierce™ Alkylating Reagents
Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.
Thermo Scientific™ Pierce™ Biotin Quantitation Kit
HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.
Thermo Scientific™ Pierce™ Dye Removal Columns
Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.
Molecular Probes™ Alexa Fluor™ 488 Azide (Alexa Fluor™ 488 5-Carboxamido-(6-Azidohexanyl), Bis(Triethylammonium Salt)), 5-isomer
The green-fluorescent Alexa Fluor™ 488 azide is reactive with terminal alkynes via a copper-catalyzed click reaction.
Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Atto 565 NHS Ester BioReagent, Suitable for Fluorescence, 70% (Coupling to Amines), MilliporeSigma™ Supelco™
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.