Crosslinking, Labeling and Protein Modification
Thermo Scientificâ„¢ Bond-Breakerâ„¢ TCEP Solution, Neutral pH
Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.
Thermo Scientificâ„¢ EZ-Linkâ„¢ Sulfo-NHS-LC-Biotin
Thermo Scientificâ„¢ EZ-Linkâ„¢ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines.
Thermo Scientificâ„¢ Pierceâ„¢ EDC, No-Weighâ„¢ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientificâ„¢ Pierceâ„¢ Alkylating Reagents
Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.
Thermo Scientificâ„¢ Pierceâ„¢ Biotin Quantitation Kit
HABA dye reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in antibodies and other proteins.
Thermo Scientificâ„¢ Pierceâ„¢ Dye Removal Columns
Affinity resin with spin columns to quickly bind and remove excess fluorescent dye from antibody (protein) solutions following labeling reactions.
Molecular Probesâ„¢ Alexa Fluorâ„¢ 488 Azide (Alexa Fluorâ„¢ 488 5-Carboxamido-(6-Azidohexanyl), Bis(Triethylammonium Salt)), 5-isomer
The green-fluorescent Alexa Fluorâ„¢ 488 azide is reactive with terminal alkynes via a copper-catalyzed click reaction.
Thermo Scientificâ„¢ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).
Atto 565 NHS Ester BioReagent, Suitable for Fluorescence, 70% (Coupling to Amines), MilliporeSigmaâ„¢ Supelcoâ„¢
Fluorescence of bioconjugates; employed in fluorescence resonance energy transfer, FRET, experiments.
MilliporeSigmaâ„¢ ALCALASEâ„¢ Enzyme, Bacillus licheniformis
Suitable for hydrolysis of proteins
Bolton-Hunter Reagent, Thermo Scientific Chemicals
CAS: 34071-95-9 Molecular Formula: C13H13NO5 Synonym: N-Succinimidyl-3-(4-hydroxyphenyl)propionate; Rudinger reagent
Invitrogenâ„¢ Alexa Fluorâ„¢ 633 NHS Ester (Succinimidyl Ester)
Alexa Fluorâ„¢ 633 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line.
Molecular Probesâ„¢ Alexa Fluorâ„¢ 700 NHS Ester (Succinimidyl Ester)
Alexa Fluor™ 700 is a bright and photostable near-IR dye that can be excited with a xenon-arc lamp, far-red diode lasers, or dye pumped lasers operating in the 675–700 nm range.