Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonyme: TBE
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonyme: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tris-Glycine, 10X Solution (Electrophoresis), Fisher BioReagents™
Tris (Tromethamine) (Granular/USP), Fisher Chemical™
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Numéro MDL: MFCD00004679 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonyme: Trishydroxymethylaminomethane,Tris Buffer,Tris,THAM,Trizma™,2-Amino-2-(hydroxymethyl)-1,3-propanediol,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tris(hydroxymethyl)aminomethane (Cryst./Certified ACS), Fisher Chemical™
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Numéro MDL: MFCD00004679 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonyme: 2-Amino-2-(hydroxymethyl)-1, 3-propanediol,Trimethylol Aminomethane,Tris Buffer,Tris,Trizma™,THAM,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
SDS-PAGE Buffer, 5X Powder, Electrophoresis, Fisher BioReagents
Tris Hydrochloride, 1M Solution (pH 7.0/Mol. Biol.), Fisher BioReagents™
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonyme: Tris-HCl CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
HEPES Buffer, 1M Solution, pH 7.3 (Molecular Biology), Fisher BioReagents™
Commonly used buffering agent
TBS, Tris Buffered Saline, 1X Solution, pH 7.4, Molecular Biology, Fisher BioReagents
MES (Fine White Crystals), Fisher BioReagents
CAS: 145224-94-8 Formule moléculaire: C6H15NO5S Poids moléculaire (g/mol): 213.248 Clé InChI: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonyme: 2-(4-Morpholino)ethane Sulfonic Acid,2-(N-Morpholino)ethanesulfonic acid hydrate CID PubChem: 16218417 Nom IUPAC: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
