Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonyme: TBE
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HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent.
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Gibco™ DPBS, no calcium, no magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™
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Gibco™ PBS, pH 7.4
PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™
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Corning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X
With low endotoxin content.
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.6
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Numéro MDL: MFCD00004679 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonyme: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
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PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
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Corning™ Cell Culture Phosphate Buffered Saline (1X)
With low endotoxin content. Without calcium and magnesium.
Gibco™ HEPES (1M)
A zwitterionic organic chemical buffering agent commonly used in cell culture media