Life Science Buffers
Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Résultats de la recherche filtrée
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent.
Poids moléculaire (g/mol) | 238.30 |
---|---|
Danger pour la santé 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
Symbole de stockage ChemAlert | Gray |
Danger pour la santé 2 | WARNING! |
Qualité | Molecular Biology |
ChEBI | CHEBI:42334 |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
Identification | Pass Test |
DNase | DNase free |
Merck Index | 15, 4689 |
Protéase | Protease free |
Température de stockage | RT |
Formule moléculaire | C8H18N2O4S |
Informations sur la solubilité | Soluble in water |
Couleur | White |
Numéro MDL | MFCD00006158 |
Nom chimique ou matériau | HEPES |
Absorbance | 0.01 max. (0.1M solution) at 280nm |
CAS | 7365-45-9 |
CID PubChem | 23831 |
Notes de qualité de la pureté | DNase-, RNase- and Protease-Free |
pH | 5.0 to 6.5 |
Clé InChI | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
Plage de pourcentage du dosage | ≥99 % |
Pourcentage de pureté | ≥99% |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonyme: TBE
Synonyme | TBE |
---|---|
CAS | 10043-35-3 |
Gibco™ DPBS, no calcium, no magnesium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Indicateur rouge de phénol | No Phenol Red |
---|---|
Type de produit | DPBS |
Stérilité | Sterile-filtered |
Classification | Animal Origin-Free |
Qualité de fabrication | cGMP-compliant under the ISO 13485 standard |
pH | 7.0 to 7.3 |
Type de solution | Dulbecco's Phosphate Buffered Saline |
Osmolalité | 270 - 300 mOsm/kg |
Forme | Liquid |
À utiliser avec (application) | Mammalian Cell Culture |
Gibco™ PBS, pH 7.4
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Concept écologique | Sustainable packaging |
---|---|
Type de produit | Phosphate Buffered Saline (PBS) |
Additif | No Sodium Pyruvate |
Qualité de fabrication | cGMP-compliant under the ISO 13485 standard |
Conditions d’expédition | Room Temperature |
Forme | Liquid |
À utiliser avec (application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
Indicateur rouge de phénol | No Phenol Red |
Couleur | Clear |
Dilution | 1 X |
Stérilité | Sterile-filtered |
Classification | Animal Origin-Free |
pH | 7.4 |
Gamme de produits | Gibco™ |
Type de solution | Phosphate Buffered Saline |
Osmolalité | 280 - 315 mOsm/kg |
PBS, Phosphate Buffered Saline, 1X Solution, pH 7.4, Fisher BioReagents™
pH | 7.3 to 7.5 |
---|---|
Point de fusion | 0°C |
Type d’emballage | Poly Cube |
Forme | Liquid |
Contenu et stockage | Room temperature |
Corning™ Cell Culture Buffers: Dulbecco's Phosphate-Buffered Salt Solution 1X
With low endotoxin content.
Dilution | 1 X |
---|---|
Type de produit | DPBS |
Couleur | Colorless |
---|---|
Note relative au nom | 1X Solution, pH 7.4 |
Synonyme | TBS |
Symbole de stockage ChemAlert | Gray |
Nom chimique ou matériau | Tris Buffered Saline |
CAS | 77-86-1 |
Qualité | Molecular Biology |
DNase | DNase free |
Point d’ébullition | 100°C |
Protéase | Protease free |
Forme physique | Liquid |
Température de stockage | RT |
Thermo Scientific Chemicals TRIS-buffered saline (TBS, 10X) pH 7.6
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Numéro MDL: MFCD00004679 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Poids moléculaire (g/mol) | 121.136 |
---|---|
Numéro MDL | MFCD00004679 |
CAS | 77-86-1 |
CID PubChem | 6503 |
ChEBI | CHEBI:9754 |
Nom IUPAC | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
Clé InChI | LENZDBCJOHFCAS-UHFFFAOYSA-N |
SMILES | C(C(CO)(CO)N)O |
Formule moléculaire | C4H11NO3 |
Qualité | Biotech |
---|---|
pH | 7.3 to 7.5 |
Type d’emballage | Poly Bottle |
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Formule moléculaire: C4H11NO3 Poids moléculaire (g/mol): 121.136 Clé InChI: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonyme: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol CID PubChem: 6503 ChEBI: CHEBI:9754 Nom IUPAC: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Poids moléculaire (g/mol) | 121.136 |
---|---|
Synonyme | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
CAS | 77-86-1 |
CID PubChem | 6503 |
ChEBI | CHEBI:9754 |
Nom IUPAC | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
Clé InChI | LENZDBCJOHFCAS-UHFFFAOYSA-N |
SMILES | C(C(CO)(CO)N)O |
Formule moléculaire | C4H11NO3 |
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
Forme | Liquid |
---|---|
Contenu et stockage | Room temperature |
Gibco™ HEPES (1M)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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A zwitterionic organic chemical buffering agent commonly used in cell culture media
Nom chimique ou matériau | Zwitterionic Organic Chemical Buffer |
---|---|
Qualité | Biochemical |
Stérilité | Sterile-filtered |
Type de solution | Buffer |
Forme physique | Liquid |
À utiliser avec (application) | Chromatin biology, mammalian cell culture |
Gibco™ ACK Lysing Buffer
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
TE Buffer, Tris-EDTA, 1X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™
Tris-EDTA (TE) is routinely used for suspending nucleic acid samples.
Note relative au nom | 1X Solution, pH 7.4 |
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Danger pour la santé 3 | May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. |
Symbole de stockage ChemAlert | Gray |
Filtré à travers | Filtered through a 5-micron filter. |
Danger pour la santé 2 | CAUTION! |
Qualité | Molecular Biology |
DNase | DNase free |
Protéase | Protease free |
Forme physique | Liquid |
Température de stockage | RT |
Couleur | Colorless |
Synonyme | TE |
Nom chimique ou matériau | Tris-EDTA |
CAS | 7732-18-5 |
pH | 7.4 to 8.1 |
Corning™ Cell Culture Phosphate Buffered Saline (1X)
With low endotoxin content. Without calcium and magnesium.
Dilution | 1 X |
---|---|
pH | 7.3 to 7.5 |
Formule | Potassium Dihydrogen Phosphate 0.144g/L, Sodium Chloride 9g/L, Disodium Phosphate 0.795g/L |
Forme | Liquid |
Contenu et stockage | 2° to 30°C |