Dissociation Reagents

Gibco™ ACK Lysing Buffer DFS Item

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

Gibco™ N-2 Supplement (100X) DFS Item

N-2 Supplement (100X)

Gibco™ L-Glutamine (200 mM) DFS Item

Ready-to-use stock solution that acts as a cell culture supplement

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ HT Supplement (100X) DFS Item

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Gibco™ 2-Mercaptoethanol DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Gibco™ Gurr Buffer Tablets DFS Item

A convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X) DFS Item

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Pluronic™ F-68 Non-ionic Surfactant (100X) DFS Item

A non-ionic surfactant used to control shear forces in suspension cultures

Gibco™ HAT Supplement (50X) DFS Item

HAT-supplemented medium is suitable for post-fusion selection against unfused or self-fused HGPRT-myeloma cells

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Gibco™ B-27™ Supplement, minus insulin DFS Item

B-27 Supplement, minus insulin

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

Gibco™ KaryoMAX™ Potassium Chloride Solution DFS Item

0.075M hypotonic solution for enlarging cells to facilitate the spreading of metaphase chromosomes for karyotyping

Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent DFS Item

StemPro Accutase Cell Dissociation Reagent

Gibco™ B-27™ Supplement (50X), minus vitamin A DFS Item

Suitable for use in most neuronal cell applications

Corning™ Cell-Tak Cell and Tissue Adhesive

Attach cells or tissue sections to many types of surfaces, including plastic, glass, metal, FEP Polymer, and biological materials. Corning™ Cell-Tak Cell and Tissue Adhesive is a specially formulated protein solution extracted from marine mussel, Mytilus edulis.

Gibco™ Poly-D-Lysine DFS Item

Poly-D-Lysine is an extracellular matrix used to facilitate neural cell adhesion to tissue culture-treated plastic and glass surfaces. When used on Nunc plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.

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