Protein Modification Reagents

Chemical agents used to modify the amino acid side chains of proteins in order to alter their native charges, block or expose reactive binding sites, inactivate functional groups, and change functional groups to create targets for crosslinking and labeling.

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Thermo Scientific™ Proteinase K Powder (≥30 U/mg)

Cleave at the carboxyl-side of aliphatic, aromatic or hydrophobic residues and digest-inactivate DNase and RNase in nucleic acid purification using this protease.

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Thermo Scientific™ Trypsin, TPCK Treated

Thermo Scientific Pierce TPCK Trypsin is a serine endoprotease that is applicable to amino acid analysis and protein sequencing, mapping and structural studies; the immobilized form allows sample separation after treatment.

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Invitrogen™ DTT, 1M

DTT, 1M

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Thermo Scientific™ Urea

Pure, crystalline urea (CH4N2O), CAS 57-13-6; denaturant with low UV-absorptivity for protein biology methods (elution, solubilization, refolding, storage).

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MilliporeSigma™ Calbiochem™ SPUTOLYSIN™ Reagent

Intended for isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum

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Thermo Scientific™ Pierce™ Alkylating Reagents

Alkylating reagents provided in single-use, pre-weighed formats to streamline protein preparation workflows.

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Specifications

Product Type	Alkylating Reagent
Product Line	Pierce

Invitrogen™ Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)

Tris-(2-Carboxyethyl)phosphine, Hydrochloride (TCEP)

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Thermo Scientific™ Pierce™ DTT (Dithiothreitol)

Reduce disulfides in biological buffers and sample loading buffers with pure, crystalline dithiothreitol (DTT), the most popular protein disulfide reducing agent.

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Thermo Scientific™ Pierce™ Sulfo-NHS-Acetate

Amine-reactive (NHS ester) compound to permanently block primary amines (e.g., protein lysine side chains) to prevent interactions or later conjugation.

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Thermo Scientific™ Bond-Breaker™ TCEP Solution, Neutral pH

Efficiently reduce protein disulfide bonds with this stable, odorless, 0.5M solution of TCEP reducing agent that is ideal for making reducing SDS-PAGE sample buffers.