Protein Modification Reagents
Thermo Scientific™ Immobilized Trypsin, TPCK Treated (Agarose Resin)
Cleave the carboxyl-side of Arg and Lys residues with trypsin that isTPCK-treated to block chymotrypsin activity; its immobilized form allows enzyme removal after digestion.
Thermo Scientific™ Pierce™ Mercaptoethylamine-HCl
Pure, crystalline 2-aminoethanethiol (2-MEA-HCl, also called cysteamine-HCl), CAS 156-57-0; selectively reduces antibody hinge-region disulfide bonds.
Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent
A three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent PEGylation of primary amines on proteins or assay surfaces.
Thermo Scientific™ Pierce™ SATP (N-succinimidyl-S-acetylthiopropionate)
Short (4.1A), amine-reactive (NHS ester) sulfhydryl-addition reagent; creates stable (protected yet exposable) -SH group for protein crosslinking strategies.
MilliporeSigma™ Dipeptidylpeptidase IV, His·Tag™, Human, Recombinant, S. frugiperda
Inhibition of DPPIV has been reported to be effective treatment for type II diabetes
MilliporeSigma™ Calbiochem™ EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated
Suitable for use in molecular biology applications
MilliporeSigma™ Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins
MilliporeSigma™ Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes
Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins
MilliporeSigma™ Endopeptidase Lys-C, Achromobacter lyticus
Specifically hydrolyzes amide and peptide ester bonds at carboxylic side of lysine and S-aminoethylcysteine residues
MilliporeSigma™ Calbiochem™ BAPTA/AM
Can be loaded into wide variety of cells, where it is hydrolyzed by cytosolic esterases and is trapped intracellularly as the active chelator BAPTA
MilliporeSigma™ Angiotensin Converting Enzyme, His·Tag™, Human, Recombinant, NSO Cells
Type I membrane protein that exists in two isoforms, somatic and germinal
MilliporeSigma™ Angiotensin Converting Enzyme-2, His·Tag™, Human, Recombinant, NSO Cells
Plays important role in regulating cardiovascular and renal functions
MilliporeSigma™ Calbiochem™ Carboxypeptidase B, Porcine Pancreas
Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides
Thermo Scientific™ Immobilized Pepsin (Agarose Resin)
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).