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Thermo Scientific™ Immobilized Pepsin (Agarose Resin)

Catalog No. PI20343
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PI20343 5 mL
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Catalog No. PI20343 Supplier Thermo Scientific™ Supplier No. LSG20343
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Description

Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.

Thermo Scientific™ Pierce Immobilized Pepsin consists of an acidic endopeptidase (pepsin) that has been immobilized onto beaded agarose resin to enable generation and purification of Fab and F(ab')2 fragments from antibodies.

Immobilized pepsin can be substituted for free pepsin in any application, and is advantageous because it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the pepsin. Immobilized pepsin is also more stable against heat-induced denaturation, resulting in longer maintenance of activity. The F(ab')2 Preparation Kit contains immobilized pepsin, other necessary reagents and an optimized protocol for human, rabbit and mouse antibody digestion.

Highlights:

  • Pepsin can cleave Fc fragments from IgG, resulting in F(ab')2 fragments
  • Broad specificity, including esterase activity
  • Easy to use - simply remove the agarose beads to stop the enzymatic digestion

Includes:

Kit contains immobilized pepsin, other necessary reagents and an optimized protocol for human, rabbit and mouse antibody digestion.

Specifications

Content And Storage Upon receipt store product at 4-8°C. Product is shipped at ambient temperature.
Form Liquid Suspension
Quantity 5 mL
Product Type Pepsin

Frequently Asked Questions (FAQs)

Can I purchase the immobilized enzymes (proteases) from the Thermo Scientific IgG Fragmentation kit separately?

Yes. The three immobilized enzymes are available in 5 mL package sizes as shown below:

• Immobilized Ficin, Cat. No. 44881
• Immobilized Papain, Cat. No. 20341
• Immobilized Pepsin, Cat. No. 20343
What is the best enzyme to use for protein digestion for mass spectrometry samples?

Trypsin (Cat. Nos. 90057, 90058) or Trypsin/LysC mix (Cat. Nos. A40007, A40009, A41007) are most commonly used for proteomic applications in order to ensure reproducibility and complete digestion. Other commonly used enzymes for purified protein characterization and unique applications include Chymotrypsin Protease, MS Grade (Cat. No. 90056), Immobilized Pepsin (Cat. No. 20343), LysN Protease, MS Grade (Cat. No. 90300), Asp-N Protease, MS Grade (Cat. No. 90053), and Glu-C Protease, MS Grade (Cat. No. 90054).

For Research Use Only. Not for use in diagnostic procedures.