Protein Modification Reagents
Thermo Scientific™ TMS(PEG)12 Branched Methyl-PEG-NHS-Ester Reagent
A three-branched, polyethylene glycol compound (3 times 12 PEG units) activated as an NHS ester for covalent PEGylation of primary amines on proteins or assay surfaces.
Thermo Scientific™ Guanidine-HCl
Pure, crystalline guanidium hydrochloride (GdnHCl, GuHCl), CAS 50-01-1; denaturant for protein biology methods (elution, solubilization, refolding, storage).
Thermo Scientific™ Pierce™ 2-Mercaptoethanol
Pure, liquid (equals 14M), beta-mercaptoethanol (bME, 2BME, 2-ME, b-mer), CAS 60-24-2; thiol reducing agent for cleaving protein disulfide bonds (cystines cysteines).
MilliporeSigma™ Dipeptidyl Peptidase IV (CD26), Porcine Kidney
Inhibition of DPPIV has been reported to be effective treatment for type II diabetes
MilliporeSigma™ Calbiochem EGTA/AM
Application in Life Science research
MilliporeSigma™ Calbiochem™ Sorbitol
Produced by reduction of glucose
MilliporeSigma™ Calbiochem™ Thrombospondin, Human Platelets
Blocks FGF-induced neovascularization of rat cornea.
Thermo Scientific™ MS(PEG)12 Methyl-PEG-NHS-Ester Reagent
Pegylate amines (-NH2 groups) on proteins (lysine residues) or assay surfaces using MS(PEG)n, amine-reactive (NHS ester), methyl-capped PEG compounds (4 to 24 EG units).
MilliporeSigma™ Aminopeptidase M, Porcine Kidney
Useful in peptide sequencing
Thermo Scientific™ Immobilized Pepsin (Agarose Resin)
Generate and purify Fab and F(ab')2 fragments from antibodies with acidic endopeptidase (pepsin) immobilized onto beaded agarose resin.
Thermo Scientific™ Pierce™ Sodium meta-Periodate
Gently oxidize glycoprotein carbohydrate sugars to create reactive aldehydes (carbonyls) for crosslinking and immobilization using NaIO4 (CAS 7790-28-5).
Thermo Scientific™ Pierce™ Hydroxylamine-HCl
NH2OH (CAS 7803-49-8) to cleave Asn-Gly peptide bonds or deprotect sulfhydryls following S-acetylthioacetate modification with SATA reagent.
Thermo Scientific™ Pierce™ HRV 3C Protease Solution Kit (2 units/μL)
Remove fusion tags from proteins with the HRV 3C cleavage sequence with this recombinant cysteine protease that is dual tagged for easy removal from the sample after cleavage.
Invitrogen™ Thiol and Sulfide Quantitation Kit
Ultrasensitive colorimetric assay for quantifying both protein and nonprotein thiols
Thermo Scientific™ NeuCode™ Lysine-080
Increase the level of multiplexing achievable with NeuCode™ amino acids for the metabolic labeling of proteins for mass spectrometry analysis.