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Immunoassay Reagents and Kits

Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.

We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.

ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.

Life Science Research ELISA Kits (3,992)
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ELISA Substrates and Reagents (62)

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1 – 15 of 3,481 results
1

Invitrogen™ SARS-CoV-2 Neutralizing Ab ELISA Kit  DFS Item

ELISA

Shipping Condition Wet Ice
Content And Storage 2°C to 8°C
Host Species Human
Conjugate HRP
Interassay CV <10%
Intraassay CV <10
Total Assay Time 1 hrs 30 mins
Sample Volume Plasma 5 μL, Serum 5 μL
Protein SARS-CoV-2 Spike Protein RBD
Test Time 40 mins
Regulatory Status RUO
Target SARS-CoV-2 Spike Protein RBD
Kit Contents Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Product Type ELISA
Sample Type Plasma,Serum
2

Invitrogen™ ProcartaPlex™ Human Liver Toxicity Panel 2, 5plex

The ProcartaPlex Human Liver Toxicity Panel 2 5plex enables hepatotoxicity research by analyzing six protein targets in a single well using Luminex xMAP technology.

3

Invitrogen™ ProcartaPlex™ Human Immune Monitoring Panel, 65plex

The ProcartaPlex Human Immune Monitoring Panel 65plex enables the study of immune function by analyzing 65 protein targets in a single well using Luminex xMAP technology.

4

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

5

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
6

Invitrogen™ Mouse IL-1 alpha ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Mouse
Conjugate FAM™
Incubation Time 1 hr.
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.3 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 0.32 to 5000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P01582
Regulatory Status RUO
Target IL-1 alpha
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 16175
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
7

Invitrogen™ Human MCP-2 ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Human
Conjugate FAM™
Incubation Time 1 hr.
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.01 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 0.0128 to 5000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P80075
Regulatory Status RUO
Target CCL8 (MCP2)
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 6355
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
8

Invitrogen™ Human IL-3 ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Human
Conjugate FAM™
Incubation Time 1 hr.
Gene Symbol IL-3; MCGF; MULTI-CSF
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.01 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 0.0128 to 5000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P08700
Regulatory Status RUO
Target IL-3
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 3562
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
9

Invitrogen™ Mouse IL-2 ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Mouse
Conjugate FAM™
Incubation Time 1 hr.
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.05 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 0.064 to 5000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P04351
Regulatory Status RUO
Target IL-2
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 16183
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
10

Invitrogen™ Mouse Periostin (Postn) ELISA Kit  DFS Item

ELISA

Protein Family Other Proteins
Host Species Mouse
Target Species Mouse
Conjugate HRP
Description Periostin (Postn) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Periostin, Osteoblast Specific Factor (Postn) in Samples Using a M
Intraassay CV <10
Detectable Analytes Periostin, osteoblast specific factor (Postn)
Total Assay Time 4 hrs 45 mins
Sample Volume Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
Assay Sensitivity 0.05 ng/mL
Research Discipline Development
For Use With (Application) ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Test Time 1 hrs 20 mins
Detection Method Colorimetric
Assay Solid-phase sandwich ELISA
Target Periostin
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: Axl, BLC, CD27 Ligand, CD30, CD30 Ligand, CD40, CXCL16, EGF, E-selectin, Fractalkine, GITR, HGF, ICAM-1, IFN-gamma, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 ra, IL-1 beta, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-17 E, IL-17 F, IL-20, IL-23, IL-28, I-TAC, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, P-selectin, Resistin, SCF, SDF-1 alpha, TPO, VCAM-1.
Kit Contents Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers
For Use With (Equipment) Colorimetric Microplate Reader
Label or Dye HRP
Calibration Range 0.05 ng/mL to 16 ng/mL
Interassay CV <12%
Gene Symbol A630052E07Rik,AI747096,OSF-2,Osf2,PLF,PN
Storage Requirements 2°C to 8°C
Assay Range 0.66 to 16 ng/mL
Accession Number Q62009
Regulatory Status RUO
Product Type ELISA
Gene ID (Entrez) 50706
Sample Type Plasma,Serum,Supernatant
Synonym A630052E07Rik, AI747096, OSF-2, Osf2, PLF, PN, peri
11

Invitrogen™ Human CD163 Uncoated ELISA Kit

ELISA

For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 9332
Assay Sensitivity μg/mL
13

Invitrogen™ Human SAA ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Human
Conjugate FAM™
Incubation Time 1 hr.
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <240 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 256 to 4,000,000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P0DJI8
Regulatory Status RUO
Target SAA
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 6288
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
14

Invitrogen™ Human CXCL1 (GRO alpha) ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Human
Conjugate FAM™
Incubation Time 1 hr.
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.01 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 0.0128 to 1000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P09341
Regulatory Status RUO
Target CXCL1 (GRO alpha)
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 2919
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™
15

Invitrogen™ Human IFN alpha ProQuantum Immunoassay Kit  DFS Item
GREENER_CHOICE

ELISA

Immunoassay Kit Format Proximity-based Immunoassay
Target Species Human
Conjugate FAM™
Incubation Time 1 hr.
Total Assay Time 2 hrs
Sample Volume Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL
Assay Sensitivity <0.05 pg/mL
Storage Requirements Frozen at -20°C
Assay Range 0.064 to 5000 pg/mL
For Use With (Application) Protein Quantitation
Test Time 30 mins
Detection Method Taqman™ Real-time PCR
Accession Number P01562
Regulatory Status RUO
Target IFN alpha
Kit Contents Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase
For Use With (Equipment) qPCR Instruments
Product Type ProQuantum
Gene ID (Entrez) 3439, 3440
Sample Type Plasma,Serum,Supernatant
Label or Dye FAM™