Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Human MCP-2 ProQuantum Immunoassay Kit
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ELISA
| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM™ |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hrs |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.01 pg/mL |
| Storage Requirements | Frozen at -20°C |
| Assay Range | 0.0128 to 5000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 mins |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P80075 |
| Regulatory Status | RUO |
| Target | CCL8 (MCP2) |
| Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 6355 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
| Protein Family | Cytokines & Receptors |
|---|---|
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IFN-G,IFN-gamma |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Storage Requirements | 2°C to 8°C |
| Research Discipline | Immunology |
| Assay Range | 0.102 to 25 ng/mL |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Assay | Solid-phase sandwich ELISA |
| Accession Number | P42161 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Specificity | This ELISA antibody pair detects canine IFN-gamma, no cross-reactivity was observed with recombinant human, mouse, rat, cotton rat, feline, porcine, equine or bovine IFN-gamma. |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 403801 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | IFN-GIFN-gamma |
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | 2°C to 8°C |
| Host Species | Human |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Total Assay Time | 1 hrs 30 mins |
| Sample Volume | Plasma 5 μL, Serum 5 μL |
| Protein | SARS-CoV-2 Spike Protein RBD |
| Test Time | 40 mins |
| Regulatory Status | RUO |
| Target | SARS-CoV-2 Spike Protein RBD |
| Kit Contents | Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum |
Invitrogen™ Tissue Extraction Reagent I
Lysis buffer for total protein extraction from tissue samples
Invitrogen™ Denaturing Cell Extraction Buffer
Suitable for use in ELISA, Western blotting, sample/assay prep
Invitrogen™ LI Silver (LIS) Enhancement Kit
Convenient, light-insensitive silver enhancement system for use with colloidal gold and NANOGOLD reagents
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IL17A |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 2 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 2.05 to 500 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | CTLA8; Ctla-8; cytokine; cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; Il17; IL-17; Il17a; IL-17 A; ILN; Interleukin; interleukin 17; Interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17 precursor; interleukin 17 A; interleukin 17 A-like; interleukin-17; Interleukin17A; interleukin-17 A; LOC100339322; R-IL-17-A |
| Regulatory Status | RUO |
| Target | IL-17 A |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 282863 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Il4 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 8 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 8 to 2,000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | B cell growth factor 1; B |
| Regulatory Status | RUO |
| Target | IL-4 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 280824 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IL21 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.41 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.41 to 100 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | CVID11; C x 2; il 21; Il21; IL-21; ILN; Interleukin; interleukin 21; interleukin 21-like; Interleukin21; interleukin-21; interleukin-21 isoform; LOC100344172; porcine interleukin-21; Za11 |
| Regulatory Status | RUO |
| Target | IL-21 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 378475 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | INS |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 4.5 μIU/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 4.5 to 300 μIU/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin |
| Regulatory Status | RUO |
| Target | Insulin |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 280829 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | CXCL10 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.12 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.123 to 30 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | 10 kDa interferon gamma-induced protein; 10 kDa interferon-gamma induced protein precursor; C Cmotif chemokine; C x C motif chemokine; C7; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; chemokine C-X-C motif ligand 10; chemokine CXC-like protein; Crg2; crg-2; CXC; CXC chemokine; CXC motif chemokine; C-X-C motif chemokine 10; C-X-C motif chemokine 10-like protein; C-X-C motif chemokine ligand 10; CXCL; CXCL10; CXCL10(1-73); gamma interferon inducible protein 10; gamma IP10; gamma-IP10; gIP-10; H-IP-10; IFI10; Inp10; interferon activated gene 10; interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; interferon-gamma-inducible protein 10; interferon-gamma-inducible protein-10; interferon-inducible cytokine IP-10; interferon-inducible protein 10; IP10; IP-10; M-IP-10; Mob1; mob-1; protein 10 from interferon (gamma)-induced cell line; protein Mob-1; Scyb10; similar to Small inducible cytokine B10 precursor (CXCL10) (10 kDa interferon-gamma induced protein) (Gamma-IP10) (IP-10); small inducible cytokine B subfamily (Cys-X-Cys) member 10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; small inducible cytokine subfamily B (Cys-X-Cys), member 10; small-inducible cytokine B10 |
| Regulatory Status | RUO |
| Target | CXCL10 (IP-10) |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 615107 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Ccl2 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 1.31 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 1.31 to 320 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | Acidic seminal fluid protein; AI323594; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; Je; MCAF; MCP1; MCP-1; MCP1A; MCP-1 A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; monocyte chemotactic protein 1 A; Monocyte secretory protein JE; monocyte-chemoattractant protein-1 precursor; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; Scya2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; SMC-CF |
| Regulatory Status | RUO |
| Target | CCL2 (MCP-1) |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 281043 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |