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Promega™ PureYield™ Plasmid Midiprep System


Isolates high-quality plasmid DNA from bacterial cultures

Manufacturer: promega™  A2496

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Catalog No. PRA2496

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Description & Specifications

Specifications

For Use With (Application) Isolates high-quality plasmid DNA from bacterial cultures
Format Spin or vacuum
Includes 3 × 315mL Cell resuspension solution, 3 × 315mL Cell lysis solution, 3 × 500mL Neutralization solution, 3 × 315mL Endotoxin removal wash, 6 × 381mL Column wash, 2 × 150mL Nuclease-free water, 300 PureYield binding columns, 300 PureYield clearing columns
Product Type Plasmid Midiprep System
Quantity 300 Preps
Sample Size 50mL
Storage Requirements Store at 22° to 25°C.
Yield 100 to 200μg

  • Designed to purify 100 to 200μg of plasmid DNA from 50mL bacterial culture
  • May also be used with up to 250mL of bacterial culture using an alternative protocol
  • Utilizes a silica-membrane column which allows for the purification of large amounts of plasmid DNA
  • Incorporates an Endotoxin Removal Wash to remove substantial amounts of protein, RNA, and endotoxin contaminants
  • Compatible with a variety of protocols—centrifugation, vacuum, or a combination of centrifugation and vacuum
  • Provides rapid purification—no need for high-speed centrifugation, gravity-drip columns, or post-elution alcohol precipitation
  • Produces purified DNA suitable for sensitive applications such as eukaryotic transfection, in vitro transcription, and coupled in vitro transcription/translation