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Promega™ PureYield™ Plasmid Midiprep System

Isolates high-quality plasmid DNA from bacterial cultures

$265.00 - $2,752.00


For Use With (Application) Isolates high-quality plasmid DNA from bacterial cultures
Yield 100 to 200μg
View More Specs
Includes:  Midiprep Systems (Mfr. Nos. A2492, A2495, A2496) include cell lysis solution, cell resuspension solution, column wash, endotoxin removal wash, neutralization solution, nuclease-free water, PureYield binding columns, PureYield clearing columns. Start-Up Kit (Mfr. No. A6740) also includes Welch™ Vacuum Pump, 110V; Vac-Man™ Laboratory Vacuum Manifold, 20-sample capacity; and four Eluator™ Vacuum Elution Devices.


U.S. Patent 6,194,562, Australian Patent 740145, Canadian Patent 2,329,067.

Catalog Number Mfr. No. Includes Quantity Price Quantity    


Midiprep System (100 preps), Vac-Man Laboratory Vacuum Manifold (20-sample capacity), 4 Eluator elution devices, Vacuum pump 100 Preps Each for $1,373.88


75mL Cell resuspension solution, 75mL Cell lysis solution, 130mL Neutralization solution, 85.3mL Endotoxin removal wash, 210mL Column wash, 25mL Nuclease-free water, 25 PureYield binding columns, 25 PureYield clearing columns 25 Preps Each for $265.00


315mL Cell resuspension solution, 315mL Cell lysis solution, 500mL Neutralization solution, 315mL Endotoxin removal wash, 2 × 381mL Column wash, 4 × 25mL Nuclease-free water, 100 PureYield binding columns, 100 PureYield clearing columns 100 Preps Each for $961.00


3 × 315mL Cell resuspension solution, 3 × 315mL Cell lysis solution, 3 × 500mL Neutralization solution, 3 × 315mL Endotoxin removal wash, 6 × 381mL Column wash, 2 × 150mL Nuclease-free water, 300 PureYield binding columns, 300 PureYield clearing columns 300 Preps Each for $2,752.00
Description & Specifications


For Use With (Application) Isolates high-quality plasmid DNA from bacterial cultures
Yield 100 to 200μg

  • Designed to purify 100 to 200μg of plasmid DNA from 50mL bacterial culture
  • May also be used with up to 250mL of bacterial culture using an alternative protocol
  • Utilizes a silica-membrane column which allows for the purification of large amounts of plasmid DNA
  • Incorporates an Endotoxin Removal Wash to remove substantial amounts of protein, RNA, and endotoxin contaminants
  • Compatible with a variety of protocols—centrifugation, vacuum, or a combination of centrifugation and vacuum
  • Provides rapid purification—no need for high-speed centrifugation, gravity-drip columns, or post-elution alcohol precipitation
  • Produces purified DNA suitable for sensitive applications such as eukaryotic transfection, in vitro transcription, and coupled in vitro transcription/translation