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TRIzol™ Reagent is a complete, ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA, and protein from a variety of biological samples.
Shipping Condition | Room Temperature |
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Final Product Type | Total RNA, DNA, Protein |
Sample Type | Bacteria,Cells,Plants,Tissue,Yeast |
Isolation Technology | Organic Extraction |
Starting Material Amount | Bacteria: ≤107 cells Cells: ≤107 Plant: ≤107 cells, 100 mg Tissue: ≤100 mg Yeast: ≤107 cells |
Purification Time | 1 hr. |
Yield | DNA: ≤7 μg RNA: ≤73 μg Protein: varies |
Elution Volume | 20 to 600 μL |
For Use With (Application) | RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern/Southern/Western blotting, in vitro translation, nuclease protection assays, nucleic acid labeling, hybridization, enzymatic assays, immunoprecipitation, gel shift assays, 2D gel electrophoresis |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Achieve accurate and precise quantification of double-stranded DNA with Qubit dsDNA HS and BR Assay Kits. These dsDNA quantification kits enable quick and selective detection of low and high abundance DNA samples, and can distinguish dsDNA from ssDNA, RNA, protein, and free nucleotides.
Shipping Condition | Room Temperature |
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Excitation/Emission | 510/527 |
For Use With (Equipment) | Qubit Fluorometer |
Product Line | Qubit |
Detection Method | Fluorescence |
RNAlater RNA Stabilization Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.
RNaseZap™ RNase Decontamination Solution is a surface decontamination solution that destroys RNases on contact. You simply spray RNaseZap™ Solution onto the surface to be decontaminated and rinse it off with RNase-free water.
Ambion Ultrapure BSA is a 'non-acetylated' BSA pure enough to use when DNA or RNA integrity is critical.
Shipping Condition | Dry Ice |
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Content And Storage | Store at –20°C. |
Form | Liquid |
Concentration | 50 mg/mL |
Product Line | Ambion™ |
Highly selective for dsDNA over RNA, the Qubit 1X dsDNA HS and BR Assay Kits offer easy-to-use formulations for the accurate quantitation of dsDNA in 100 and 500 assay formats. Lambda Standards provide sufficient material for up to 500 samples.
Green Features | Fewer resources used, Less waste |
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Product Line | Qubit |
Pre-mixed, pH-adjusted, sterile-filtered solution
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.
Product Type | DTT (Dithiothreitol) |
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Concentration | 0.1 M |
Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent.
Shipping Condition | Room Temperature |
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Excitation/Emission | 500/525 |
No. of Reactions | 200 Assays (2 mL assay volume) |
Quantitation Range | 50 pg to 2 μg |
For Use With (Equipment) | Microplate Reader |
Product Line | PICOGREEN, Quant-iT |
Detection Method | Fluorescence |
Thermo Scientific GeneJET Plasmid Miniprep Kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column.
Target | Plasmid DNA |
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Format | Spin Column |
Product Type | Plasmid MiniPrep Kit |
Sample Type | Bacteria |
Isolation Technology | Silica Column |
Column Type | Spin Column |
Scale | Mini |
High-throughput Compatibility | Compatible (Vacuum manifold) |
For Use With (Application) | PCR, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, In Vitro Transcription |
Test Time | <14 min |
Product Line | GeneJET |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Thermo Scientific GeneJET Gel Extraction Kit is designed for rapid and efficient purification of DNA fragments from standard or low-melting point agarose gels run in either TAE or TBE buffer.
Content And Storage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
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Column Type | Spin Column |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
Test Time | 15 min. |
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
Pre-mixed, pH-adjusted, sterile-filtered solutions
For highly selective quantitation of double-stranded DNA over RNA, the Quant-iT dsDNA Assay Kits, high sensitivity and broad range, produce fluorescence signals that are linear in the ranges of 0.2-100 ng and 2-1000 ng of DNA, respectively.
Shipping Condition | Room Temperature |
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No. of Reactions | 1000 Assays (200 μL assay volume) |
For Use With (Equipment) | Microplate Reader |
Quantity | 1 kit |
Product Line | Quant-iT |
Detection Method | Fluorescence |
The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from E.coli in about 30 minutes, using silica membrane columns.
Shipping Condition | Room Temperature |
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Content And Storage | • Resuspension buffer • RNase A (20 mg/mL) • Lysis buffer • Precipitation buffer • Wash buffers • TE buffer • Spin columns in wash tubes • Elution tubes |
Final Product Type | Plasmid DNA |
Isolation Technology | Silica Spin Column |
Column Type | Spin Column |
Concentration | 20 mg/mL |
High-throughput Compatibility | Not High-throughput Compatible (Manual) |
For Use With (Application) | Next-Generation Sequencing, Cloning, Sequencing, Nucleic Acid Labeling, PCR, Transformation |
Test Time | 30 to 45 min. |
Prep Scale | < 100 μg (Small-Scale) Plasmid DNA |
Target | Plasmid DNA |
Format | Spin Column |
Product Type | Quick Plasmid MiniPrep Kit |
Sample Type | Bacterial Culture |
Product Line | PureLink™ |