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Promega™ E. coli S30 T7 High-Yield Protein Expression System DFS Item

The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500ug/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.

Manufacturer:  Promega™ L1115

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Catalog No. PRL1115

Includes: T7 S30 Extract, an optimized S30 Premix Plus provides all other required components, including amino acids, rNTPs, tRNAs, an ATP-regenerating system, IPTG and appropriate salts to express high levels of recombinant proteins. Also includes S30 T7 Control DNA, a control DNA template.



All components are optimized for protein expression, resulting in greater stability and enhanced expression of target proteins.

  • Achieve high protein expression: up to 500μg/mL of protein for multiple applications Process completed in only one hour, not days, as with cell-based expression Convenient screening protocol for high-throughput protein expression Choose from several detection methods: Coomassie™ staining, or incorporation of a fluorescence- or biotinylated-modified tRNA

Protein:protein interactions, Protein:DNA interactions, Enzyme activity, Antibody production



Protein-Protein interactions, Protein-DNA interactions, Enzyme activity, Antibody production
8 Reactions
1 hr.
S30 T7 high yield Protein expression system


U.S. Patents 5,292,658, and 5,418,155; Canadian Patent 2,105,984 and related patents.