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SuperScript™ VILO™ cDNA Synthesis Kit DFS Item

Provides the high-temperature capability of SuperScript III Reverse Transcriptase (RT) in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR)

Manufacturer: life technologies  11754050

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Catalog No. 11754050

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Description & Specifications


For Use With (Application) First-Strand cDNA Synthesis, qRT-PCR
Format SuperMix or Master Mix
Includes The SuperScript VILO™ cDNA Synthesis Kit is supplied with 5X VILO™ Reaction Mix (200μL) and a 10X SuperScript III Enzyme Blend (100μL), which contains: SuperScript III RT, RNaseOUT™ Recombinant Ribonuclease Inhibitor, and a proprietary helper protein
Primer Random Primers
Quantity 50 reactions
Sample Type RNA
Storage Requirements Store at -20deg.C upon receipt
Temperature (English) Operating 107.6deg.F
Temperature (Metric) Operating 42deg.C

VILO is an acronym for variable input, linear output; and the SuperScript VILO cDNA Synthesis Kit provides the most consistent results for RT experiments, with unprecedented linearity across the broadest range of input material.

  • Using the SuperScript VILO cDNA Synthesis Kit This formulation provides enhanced cDNA synthesis efficiency and can be used with very low and very high amounts of input RNA (up to 2.5μg total RNA in a 20μL reaction), giving a linear response in message abundance as measured by qPCR. The 10X SuperScript Enzyme Mix includes SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein
  • SuperScript III RT SuperScript III RT is an engineered version of M-MLV RT with reduced RNase H activity and increased thermal stability. The enzyme can be used to synthesize cDNA at a temperature range of 42° to 60°C. Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA. RNaseOUT Recombinant Ribonuclease Inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
  • Optimized buffer The 5X VILO Reaction Mix includes random primers, MgCl2, and dNTPs in a buffer formulation that has been optimized for qRT-PCR

  • Optimal reaction temperature: 42°C

PCR & Real-Time PCR, Reverse Transcription