
cDNA Synthesis Kits and Master Mixes
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Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
Type de réactif | Reverse Transcription |
---|---|
Vitesse de réaction | 120 min. |
Format | Kit |
Format de réaction | Separate components |
Type de produit final | First-Strand cDNA |
Quantité | Each |
Activité de la ribonucléase H | Yes |
Concentration | 50 U/μL |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Conditions d’expédition | Dry Ice |
Transcriptase inverse | MultiScribe |
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
GC-Rich PCR Performance | High |
---|---|
Température de réaction optimale | 50°C |
Type de réactif | Reverse Transcription |
Vitesse de réaction | 10 min. |
Format | Kit |
Format de réaction | Separate components |
Type de produit final | First-Strand cDNA |
Quantité | Each |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Taille (produit final) | Up to 12.3 kb |
Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ VILO™ Master Mix
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.
GC-Rich PCR Performance | High |
---|---|
Méthode de détection | qPCR |
Vitesse de réaction | 60 min. |
Format de réaction | Master Mix |
Type de produit final | First-Strand cDNA |
Activité de la ribonucléase H | Reduced |
Concentration | 5X |
Conditions d’expédition | Dry Ice |
À utiliser avec (application) | Real Time PCR (qPCR) |
Température de réaction optimale | 42°C |
Type de réactif | Reverse Transcription |
Format | Master Mix |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Taille (produit final) | Up to 10 kb |
Transcriptase inverse | SuperScript III |
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Température de réaction optimale | 37°C |
---|---|
Type de réactif | Reverse Transcription |
Vitesse de réaction | 30 to 60 min. |
Format | Kit |
Format de réaction | Premixed Components |
Type de produit final | First-Strand cDNA |
Activité de la ribonucléase H | Yes |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Taille (produit final) | Up to 7 kb |
Conditions d’expédition | Dry Ice |
Transcriptase inverse | MultiScribe |
À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
GC-Rich PCR Performance | High |
---|---|
Méthode de détection | qPCR |
Vitesse de réaction | 10 min. |
Format de réaction | Master Mix |
Type de produit final | First-Strand cDNA |
Activité de la ribonucléase H | Reduced |
Concentration | 5X |
Conditions d’expédition | Dry Ice |
À utiliser avec (application) | Real Time PCR (qPCR) |
Température de réaction optimale | 50°C |
Type de réactif | Reverse Transcription |
Format | Master Mix |
Quantité | Each |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Taille (produit final) | Up to 10 kb |
Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
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The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
GC-Rich PCR Performance | High |
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Concept écologique | Less Hazardous, Less Waste |
Vitesse de réaction | 10 min. |
Format de réaction | Premixed Components |
Type de produit final | First-Strand cDNA |
Activité de la ribonucléase H | Reduced |
Conditions d’expédition | Dry Ice |
À utiliser avec (application) | RT-PCR,Real Time PCR (qPCR) |
Température de réaction optimale | 50°C |
Type de réactif | Reverse Transcription |
Format | Kit |
Technique | Reverse Transcription |
Matériau de départ | Cells |
Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit
Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis
Température de réaction optimale | 42°C |
---|---|
Format | Kit |
Méthode d’étiquetage | Direct Labeling |
Type de produit | cDNA Synthesis Kit |
Gamme de produits | SuperScript™ |
Type d’échantillon | RNA |
Type de produit final | cDNA (Double-Stranded) |
Conditions d’expédition | Wet Ice |
Transcriptase inverse | SuperScript™ II |
À utiliser avec (application) | Microarray Analysis |
Thermo Scientific™ Verso cDNA Synthesis Kit
The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.
Température de réaction optimale | 42°C |
---|---|
Type de réactif | Reverse Transcription |
Vitesse de réaction | 30 min. |
Format | Kit |
Format de réaction | Separate components |
Type de produit final | First-Strand cDNA |
Activité de la ribonucléase H | Yes |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Taille (produit final) | Up to 11 kb |
Conditions d’expédition | Wet or Dry Ice |
Transcriptase inverse | Verso |
À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR
The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
GC-Rich PCR Performance | High |
---|---|
Méthode de détection | qPCR |
Vitesse de réaction | 60 min. |
Format de réaction | Master Mix |
Type de produit final | First-Strand cDNA |
Activité de la ribonucléase H | Reduced |
Conditions d’expédition | Dry Ice |
À utiliser avec (application) | Real Time PCR (qPCR) |
Température de réaction optimale | 42°C |
Type de réactif | Reverse Transcription |
Format | Kit |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Taille (produit final) | Up to 10 kb |
Transcriptase inverse | SuperScript III |
Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
Format de réaction | Separate Components |
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Type de produit | cDNA Synthesis Kit |
Type de produit final | Double Stranded cDNA |
À utiliser avec (application) | Cloning |
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
Température de réaction optimale | 42°C |
---|---|
Type de réactif | Reverse Transcription |
Format | Kit |
Format de réaction | Separate components |
Type de produit final | First-Strand cDNA |
Activité de la ribonucléase H | Yes |
Concentration | 50 U/μL |
Technique | Reverse Transcription |
Matériau de départ | RNA |
Taille (produit final) | Up to 5 kb |
Conditions d’expédition | Dry Ice |
Transcriptase inverse | MultiScribe |
À utiliser avec (application) | Real Time PCR (qPCR) |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
Invitrogen™ SuperScript™ III First-Strand Synthesis System
The SuperScript III First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA.
Invitrogen™ SuperScript™ IV Template Switching RT Master Mix
Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.
GC-Rich PCR Performance | High |
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Type de réactif | Reverse Transcription |
Vitesse de réaction | 30 min. |
Format | Master Mix |
Type de produit final | First-Strand cDNA |
Concentration | 4X |
Technique | Template Switching Reverse Transcription |
Matériau de départ | Cells or RNA |