accessibility menu, dialog, popup

UserName

cDNA Synthesis Kits and Master Mixes

A variety of full kits and master mixes that facilitate first strand cDNA synthesis reactions and processes.
For Use With (Application) 
  • (3)
  • (2)
  • (1)
  • (2)
  • (2)
  • (1)
  • (5)
  • (1)
  • (25)
  • (44)
  • (1)
  • (1)
Reaction Speed 
  • (10)
  • (4)
  • (23)
  • (2)
  • (4)
  • (10)
  • (2)
GC-Rich PCR Performance 
  • (43)
Concentration 
  • (3)
  • (6)
  • (9)
Detection Method 
  • (11)
  • (2)

Filtered Search Results

Products from some of our suppliers do not display in filtered search results. Please clear all filters to see these products.

Narrow Results

Narrow Results

category

special offers

  • (7)

brands

  • (7)
  • (2)
  • (35)
  • (19)

special interests

  • (2)

For Use With (Application)

  • (3)
  • (2)
  • (1)
  • (2)
  • (2)
  • (1)
  • (5)
  • (1)
  • (25)
  • (44)
  • (1)
  • (1)

Reaction Speed

  • (10)
  • (4)
  • (23)
  • (2)
  • (4)
  • (10)
  • (2)

GC-Rich PCR Performance

  • (43)

Concentration

  • (3)
  • (6)
  • (9)

Detection Method

  • (11)
  • (2)

Form

  • (2)

Label or Dye

  • (2)
  • (2)
  • (2)
  • (2)
  • (2)

PCR Method

  • (1)
  • (2)
  • (2)
  • (1)
  • (2)
  • (2)

Search Within Results
Keyword Search:
1 – 15 of 27 results
1

Thermo Scientific™ Verso cDNA Synthesis Kit

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.

Shipping Condition Wet or Dry Ice
Format Kit
Reverse Transcriptase Verso
Size (Final Product) Up to 11 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
2

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit  DFS Item

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Kit
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
3

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Quantity Each
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Format Master Mix
Size (Final Product) Up to 20 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
4

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
5

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima
Final Product Type First-Strand cDNA
Quantity Each
For Use With (Application) Real Time PCR (qPCR)
Format Kit
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
6

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase RevertAid H Minus
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C to 45°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
7

Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit  DFS Item

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Shipping Condition Wet Ice
Format Kit
Reverse Transcriptase SuperScript™ II
Final Product Type cDNA (Double-Stranded)
Product Type cDNA Synthesis Kit
Sample Type RNA
Labeling Method Direct Labeling
Optimal Reaction Temperature 42°C
For Use With (Application) Microarray Analysis
Product Line SuperScript
8

Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR  DFS Item

The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

9

Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents  DFS Item

The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.

10

Invitrogen™ SuperScript™ IV VILO™ Master Mix  DFS Item

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Quantity Each
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
11

Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit  DFS Item

The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).

Shipping Condition Dry Ice
Polymerase Platinum SuperFi U DNA Polymerase
GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 10 kb
Final Product Type PCR Amplified cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Optimal Reaction Temperature 50°C
Technique Reverse Transcription, Preamplification
Starting Material Cells or RNA
For Use With (Application) Next-Generation Sequencing (NGS),Real Time PCR (qPCR)
12

Invitrogen™ Second Strand cDNA Synthesis Kit  DFS Item

Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Reaction Format Separate Components
Final Product Type Double Stranded cDNA
Product Type cDNA Synthesis Kit
For Use With (Application) Cloning
13

Thermo Scientific™ Maxima H Minus First Strand cDNA Synthesis Kit

Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit, available with or without dsDNase, is a complete system for highly efficient synthesis of first strand cDNA.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Quantity Each
Format Kit
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
14

Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit  DFS Item

The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 120 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Quantity 1 Ea.
Technique Reverse Transcription
Concentration 50 U/μL
Starting Material RNA
15

Invitrogen™ SuperScript™ IV Template Switching RT Master Mix

Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.

GC-Rich PCR Performance High
Format Master Mix
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Technique Template Switching Reverse Transcription
Concentration 4X
Starting Material Cells or RNA