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  7. Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit

Catalog No. p-4926559
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11754250 250 Reactions
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Catalog No. 11754050 Supplier Invitrogen™ Supplier No. 11754050
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Description

Includes

5X VILO Reaction Mix (200μL) and a 10X SuperScript III Enzyme Blend (100μL), which contains: SuperScript III RT, RNaseOUT Recombinant Ribonuclease Inhibitor, and a proprietary helper protein

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes. The enzyme blend contains SuperScript III Reverse Transcriptase (RT), a genetically engineered MMLV RT that has reduced RNase H activity and improved thermostability for highly efficient cDNA synthesis.

The SuperScript VILO cDNA Synthesis Kit can be used to synthesize cDNA from for a wide range of input RNA amounts. This kit provides the high temperature capability of SuperScript III Reverse Transcriptase in an optimized format for generating first-strand cDNA for use in RT–qPCR.

Notes
• For superior first-strand cDNA synthesis in two-step RT-qPCR, try SuperScript IV VILO Master Mix (Cat. No. 11756050) or SuperScript IV VILO Master Mix with ezDNase Enzyme (Cat. No. 11766050).
• This kit is included with EXPRESS Two-Step RT–qPCR kits. Reagents are provided for 50 or 250 20-μL cDNA synthesis reactions.

Order Info

Shipping Condition: Dry ice

Specifications

Content And Storage

• 5X VILO Reaction Mix, 200 μL
• 10X SuperScript III Enzyme Blend, 100 μL

Store at –5 to –30°C.
Detection Method qPCR
Format Kit
GC-Rich PCR Performance High
Reaction Speed 60 min.
Technique Reverse Transcription
Optimal Reaction Temperature 42°C
Quantity 50 reactions
Reverse Transcriptase SuperScript III
Ribonuclease H Activity Reduced
Shipping Condition Dry Ice
For Use With (Application) Real Time PCR (qPCR)
Final Product Type First-Strand cDNA
No. of Reactions 50 Reactions
Reaction Format Master Mix
Reagent Type Reverse Transcription
Size (Final Product) Up to 10 kb
Starting Material RNA
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For Research Use Only. Not for use in diagnostic procedures.