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Reverse Transcription Products

A variety of kits, reagents, and mixes suitable for reverse transcription processes. Products include one-step RT-PCR reagent, cDNA synthesis kits and master mixes, and standalone reverse transcriptases.
cDNA Synthesis Kits and Master Mixes (63)
Reverse Transcriptases (24)
One-Step Reverse Transcription PCR Reagents (12)

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1

Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase  DFS Item

The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type PCR Amplified cDNA
PCR Method 1-step RT-PCR
Detection Method Gel Electrophoresis
Polymerase Platinum Taq High Fidelity
Format One-Step RT-PCR System
Size (Final Product) Up to 10 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Hot Start Built-In Hot Start
Optimal Reaction Temperature 50°C
Technique 1-Step RT-PCR
Starting Material RNA
2

Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System  DFS Item

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

GC-Rich PCR Performance High
Format 1-step RT-PCR Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 13 kb
Reaction Format Master Mix
Reagent Type Reverse Transcription
Hot Start Built-in Hot Start
Optimal Reaction Temperature 50°C to 65°C
Technique 1-step RT-PCR
Starting Material RNA
PCR Method 1-step RT-PCR
Multiplex Capability Yes
Detection Method Gel Electrophoresis
3

Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase  DFS Item

SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.

4

Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR  DFS Item

The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

5

Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents  DFS Item

The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.

6

Invitrogen™ SuperScript™ IV VILO™ Master Mix  DFS Item

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Quantity Each
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
7

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents  DFS Item

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Size (Final Product) Up to 5 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Concentration 50 U/μL
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR)
8

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima
Final Product Type First-Strand cDNA
Quantity Each
For Use With (Application) Real Time PCR (qPCR)
Format Kit
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
9

Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit  DFS Item

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Shipping Condition Wet Ice
Format Kit
Reverse Transcriptase SuperScript™ II
Final Product Type cDNA (Double-Stranded)
Product Type cDNA Synthesis Kit
Sample Type RNA
Labeling Method Direct Labeling
Optimal Reaction Temperature 42°C
For Use With (Application) Microarray Analysis
Product Line SuperScript
10

Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit  DFS Item

The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).

Shipping Condition Dry Ice
Polymerase Platinum SuperFi U DNA Polymerase
GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 10 kb
Final Product Type PCR Amplified cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Optimal Reaction Temperature 50°C
Technique Reverse Transcription, Preamplification
Starting Material Cells or RNA
For Use With (Application) Next-Generation Sequencing (NGS),Real Time PCR (qPCR)
11

Invitrogen™ Second Strand cDNA Synthesis Kit  DFS Item

Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.

Reaction Format Separate Components
Final Product Type Double Stranded cDNA
Product Type cDNA Synthesis Kit
For Use With (Application) Cloning
12

Invitrogen™ SuperScript™ VILO™ Master Mix  DFS Item

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
13

Invitrogen™ SuperScript™ IV Template Switching RT Master Mix

Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.

GC-Rich PCR Performance High
Format Master Mix
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Technique Template Switching Reverse Transcription
Concentration 4X
Starting Material Cells or RNA
14

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit  DFS Item

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Size (Final Product) Up to 7 kb
Reaction Format Premixed Components
Final Product Type First-Strand cDNA
Reaction Speed 30 to 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 37°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
15

Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit  DFS Item

The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.

GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript III
Size (Final Product) Up to 4.5 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material Cells
For Use With (Application) Real Time PCR (qPCR)