Reverse Transcription Products
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Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
| Température de réaction optimale | 37°C |
|---|---|
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 30 to 60 min. |
| Format | Kit |
| Format de réaction | Premixed Components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 7 kb |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | MultiScribe |
| À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
| Type de réactif | Reverse Transcription |
|---|---|
| Vitesse de réaction | 120 min. |
| Format | Kit |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Quantité | 1 Ea. |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Concentration | 50 U/μL |
| Matériau de départ | RNA |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | MultiScribe |
Thermo Scientific™ Maxima H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima H Minus Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M-MuLV RT.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | 15 to 30 min. |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Température de réaction optimale | 50°C to 55°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 20 kb |
| Transcriptase inverse | Maxima H Minus |
Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit
Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis
| Température de réaction optimale | 42°C |
|---|---|
| Format | Kit |
| Méthode d’étiquetage | Direct Labeling |
| Type de produit | cDNA Synthesis Kit |
| Gamme de produits | SuperScript |
| Type d’échantillon | RNA |
| Type de produit final | cDNA (Double-Stranded) |
| Conditions d’expédition | Wet Ice |
| Transcriptase inverse | SuperScript™ II |
| À utiliser avec (application) | Microarray Analysis |
Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR
The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Gel Electrophoresis |
| Vitesse de réaction | 30 min. |
| Format de réaction | Premixed Components |
| Type de produit final | PCR Amplified cDNA |
| Activité de la ribonucléase H | Reduced |
| Méthode PCR | 1-step RT-PCR |
| Conditions d’expédition | Dry Ice |
| Polymérase | Platinum Taq DNA Polymerase |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | One-Step RT-PCR System |
| Hot Start | Built-In Hot Start |
| Technique | 1-Step RT-PCR |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 4.5 kb |
| Transcriptase inverse | SuperScript III |
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
| Capacité multiplex | Yes |
|---|---|
| GC-Rich PCR Performance | High |
| Température de réaction optimale | 50°C to 65°C |
| Méthode de détection | Gel Electrophoresis |
| Type de réactif | Reverse Transcription |
| Format | 1-step RT-PCR Kit |
| Format de réaction | Master Mix |
| Hot Start | Built-in Hot Start |
| Méthode PCR | 1-step RT-PCR |
| Technique | 1-step RT-PCR |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 13 kb |
| Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents
The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.
Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit
The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).
| Polymérase | Platinum SuperFi U DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 30 min. |
| Format | Kit |
| Type de produit final | PCR Amplified cDNA |
| Technique | Reverse Transcription, Preamplification |
| Matériau de départ | Cells or RNA |
| Taille (produit final) | Up to 10 kb |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | SuperScript IV |
| À utiliser avec (application) | Next-Generation Sequencing (NGS),Real Time PCR (qPCR) |
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | qPCR |
| Vitesse de réaction | 10 min. |
| Format de réaction | Master Mix |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 5X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Real Time PCR (qPCR) |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | Master Mix |
| Quantité | Each |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 10 kb |
| Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | qPCR |
| Vitesse de réaction | 60 min. |
| Format de réaction | Master Mix |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Real Time PCR (qPCR) |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Kit |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 10 kb |
| Transcriptase inverse | SuperScript III |
Invitrogen™ SuperScript™ VILO™ Master Mix
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | qPCR |
| Vitesse de réaction | 60 min. |
| Format de réaction | Master Mix |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 5X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Real Time PCR (qPCR) |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Master Mix |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 10 kb |
| Transcriptase inverse | SuperScript III |
Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
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The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Less Hazardous, Less Waste |
| Vitesse de réaction | 10 min. |
| Format de réaction | Premixed Components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | RT-PCR,Real Time PCR (qPCR) |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | Kit |
| Technique | Reverse Transcription |
| Matériau de départ | Cells |
| Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR
SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).
| GC-Rich PCR Performance | High |
|---|---|
| Température de réaction optimale | 50°C |
| Méthode de détection | qPCR |
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 30 min. |
| Format | Super Mix |
| Format de réaction | Master Mix |
| Type de produit final | First-Strand cDNA |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | SuperScript III |
| À utiliser avec (application) | Real Time PCR (qPCR) |
Invitrogen™ MultiScribe™ Reverse Transcriptase
MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.