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GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

$208.32 - $753.92

Specifications

Product Type HisTrap FF Crude Column
Material Polypropylene
Particle Size 45 to 165μm
pH Range 3 to 12
Pressure 3 bar
View More Specs
Products
Catalog Number Mfr. No. Flow Rate Quantity Price Quantity    

45-000-335

 
ge healthcare
17-5286-01
<20mL/min. 5 x 5mL Pack of 5 Each for $753.92

45-000-000

 
ge healthcare
11-0004-58
<4mL/min. 60mL Pack of 5 Each for $208.32
Description & Specifications

Specifications

Product Type HisTrap FF Crude Column
Material Polypropylene
Particle Size 45 to 165μm
pH Range 3 to 12
Pressure 3 bar
Storage Requirements 4° to 30°C
Support Type 6% Cross-linked Agarose

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.