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GE Healthcare HisTrap™ FF Crude Prepacked Columns

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. GE Healthcare Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

$57.21 - $789.93

Specifications

Product Type HisTrap FF Crude
Material Polypropylene
Particle Size 45 to 165μm
pH Range 3 to 12
Pressure 5 bar [0.5 MPa] (70 psi)
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity  
Catalog Number Mfr. No. Quantity Flow Rate Price Quantity  
45-000-335 View Documents GE Healthcare
17528601
5 x 5mL < 20 mL/min Pack of 5 Each for $789.93
 
45-000-000 View Documents GE Healthcare
11000458
60mL < 4 mL/min Pack of 5 Each for $219.09
 
45-000-507 View Documents GE Healthcare
29048631
1mL < 4 mL/min Each for $57.21
 
Description

Description

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Specifications

HisTrap FF Crude
45 to 165μm
5 bar [0.5 MPa] (70 psi)
6% Cross-linked Agarose
Polypropylene
3 to 12
4° to 30°C