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Cytiva HisTrap™ FF Crude Prepacked Columns
Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
Supplier: Cytiva 11000458
Description
- Prepacked with Ni Sepharose™ 6 Fast Flow media
- Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
- Maximum pressure: 3 bar
- Column material: polypropylene
- Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
- 1 and 5mL columns
Media
- Matrix: 6% cross-linked agarose
- Average particle size: 90μm
- pH stability working range: 3 to 12
1mL columns
- Bed dimensions: 7 x 25mm
- Flow velocity: <4mL/min.
5mL columns
- Bed dimensions: 16 x 25mm
- Flow velocity: <20mL/min.
Specifications
| 1 mL | |
| 7 mm | |
| <4 mL/min. | |
| Polypropylene | |
| 6% Cross-linked Agarose | |
| 5 bar (0.5 MPa, 70 psi) | |
| 60 mL | |
| Pre-packed Column |
| 0.2 in. | |
| HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed. | |
| Sodium Phosphate-buffered Saline, pH 7.4 and 0.5X concentration of proprietary Y-PER reagent additives | |
| 3 to 12 | |
| 4°C to 30°C | |
| 45 to 165 μm | |
| Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes |