missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Cytiva HisTrap™ FF Crude Prepacked Columns

Cytiva HisTrap™ FF Crude Prepacked Columns

Catalog No. 45000000
Change view
Click to view available options
Quantity:
1 mL
5 x 5 mL
60 mL
Flow Rate:
<20 mL/min.
<4 mL/min.
3 product options available for selection
Product selection table with 3 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity Flow Rate
45000000 60 mL <4 mL/min.
45000335 5 x 5 mL <20 mL/min.
45000507 1 mL <4 mL/min.
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 3 options available.
3 options
Catalog No. 45000000 Supplier Cytiva Supplier No. 11000458
Only null left

Description

Purify histidine-tagged proteins directly from homogenized, unclarified cell lysates. Cytiva Life Sciences™ HisTrap™ FF Crude Prepacked Columns offer the convenience of simple, one-step purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

  • Prepacked with Ni Sepharose™ 6 Fast Flow media
  • Binding capacity approximately 40mg/mL medium with negligible Ni2+ leakage
  • Maximum pressure: 3 bar
  • Column material: polypropylene
  • Compatible with wide variety of reducing agents, detergents, denaturants, and other additives
  • 1 and 5mL columns

Media
  • Matrix: 6% cross-linked agarose
  • Average particle size: 90μm
  • pH stability working range: 3 to 12

1mL columns

  • Bed dimensions: 7 x 25mm
  • Flow velocity: <4mL/min.

5mL columns
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.

Specifications

Bed Volume 1 mL
Diameter (English) Inner 0.2 in.
Diameter (Metric) Inner 7 mm
Description HisTrap FF Crude columns are high binding capacity IMAC columns for his-tagged protein purification when scalability is needed.
Flow Rate <4 mL/min.
Kit Contents Sodium Phosphate-buffered Saline, pH 7.4 and 0.5X concentration of proprietary Y-PER reagent additives
Material Polypropylene
pH Range 3 to 12
Support Type 6% Cross-linked Agarose
Content And Storage 4°C to 30°C
Max. Operating Pressure 5 bar (0.5 MPa, 70 psi)
Particle Size 45 to 165 μm
Quantity 60 mL
For Use With (Application) Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes
Type Pre-packed Column
Show More Show Less