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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents 
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
$1290.00 - $12700.00
Specifications
| Concentration | 50 U/μL |
|---|---|
| Format | Kit |
| Technique | Reverse Transcription |
| Optimal Reaction Temperature | 42°C |
| Reverse Transcriptase | MultiScribe |
Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|
N8080234
|
Applied Biosystems™
N8080234 |
200 Reactions at 10 μL |
Each for $1,290.00
|
|
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|
4304134
|
Applied Biosystems™
4304134 |
2000 Reactions at 10 μL |
N/A
|
N/A | |||||
| Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance. | |||||||||
Description
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. These reagents are designed for reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The reagents in this kit facilitate two-step RT-PCR, which is performed in two separate reactions and is useful when detecting multiple transcripts from a single cDNA reaction or when storing a portion of the cDNA.
Advantages of TaqMan Reverse Transcription Reagents
- Contains MultiScribe Reverse Transcriptase, a recombinant Moloney murine leukemia virus (MMLV) reverse transcriptase
- Includes random hexamers and oligo (dT)16 for flexibility
- Individual components provide versatility in assay setup
- Compatible with two-step RT-PCR reactions
Using TaqMan Reverse Transcription Reagents
- TaqMan Reverse Transcription Reagents provide all the necessary components for reverse transcription of RNA to cDNA
- For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used
- The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems
Specifications
| 50 U/μL | |
| Reverse Transcription | |
| MultiScribe | |
| Dry Ice | |
| First-Strand cDNA | |
| Reverse Transcription | |
| RNA |
| Kit | |
| 42°C | |
| Yes | |
| Real Time PCR (qPCR) | |
| Separate components | |
| Up to 5 kb |
For Research Use Only. Not for use in diagnostic procedures.