Please login to your online account to display your discounted pricing

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents DFS Item

For use with reverse transcriptase-PCR (RT-PCR)

$672.00 - $6,342.00

Specifications

Sample Type RNA
View More Specs
Includes:  MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

N8080234

DFS Item
 
applied biosystems™
N8080234
200 reactions Each for $672.00

4304134

DFS Item
 
life technologies
4304134
2000 reactions Each for $6,342.00
Description & Specifications

Specifications

Sample Type RNA

Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

  • Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
  • Random hexamers and oligo (dT)16 included for flexibility
  • Individual components provide versatility in assay set-up
    •Compatible with one-step or two-step RT-PCR reactions
  • Optimal reaction temperature: 42°C
  • Final product size: 5kb or less

Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems. Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Applications:

PCR & Real-Time PCR, Reverse Transcription