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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents DFS Item

For use with reverse transcriptase-PCR (RT-PCR)

$694.00 - $6,500.00


Sample Type RNA
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For Research Use Only. Not for use in diagnostic procedures.

Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

Products ${productFamilyLength}
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
N8080234 Applied Biosystems™
200 reactions Each for $694.00
4304134 Life Technologies
2000 reactions Each for $6,500.00


Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

  • Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
  • Random hexamers and oligo (dT)16 included for flexibility
  • Individual components provide versatility in assay set-up
    •Compatible with one-step or two-step RT-PCR reactions
  • Optimal reaction temperature: 42°C
  • Final product size: 5kb or less

Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems. Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.


PCR & Real-Time PCR, Reverse Transcription

Description & Specifications