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Applied Biosystems™ TaqMan™ Reverse Transcription Reagents DFS Item

For use with reverse transcriptase-PCR (RT-PCR)

$1,058.00 - $1,058.00

Specifications

Concentration 50 U/μL
Final Product Type First-Strand cDNA
Format Reverse Transcription Reagent
PCR Method RT-PCR, RT-qPCR
Reaction Format Separate components
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Includes: MultiScribe™ Reverse Transcriptase (100μL at 50U/μL)

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
N8080234 DFS Item
View Documents Promotion Details
Applied Biosystems™
N8080234
200 Reactions
Each for $1,058.00
 
4304134 DFS Item
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Applied Biosystems™
4304134
2,000 Reactions N/A
N/A
Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.
Description

Description

Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

  • Contain MultiScribe Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
  • Random hexamers and oligo (dT)16 included for flexibility
  • Individual components provide versatility in assay set-up
  • Compatible with one-step or two-step RT-PCR reactions
  • Optimal reaction temperature: 42°C
  • Final product size: 5kb or less

Using TaqMan Reverse Transcription Reagents TaqMan Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.

Built-in normalization The TaqMan reverse transcription reagents contain a passive internal reference to normalize non-PCR-related fluorescence fluctuations. Normalizing with a passive internal reference minimizes well-to-well variability that can result from a variety of causes, such as pipetting errors and sample evaporation. The passive reference is also essential for accurate results when multiple probes (with different reporter dyes) are combined in a single tube.

Applications:
PCR & Real-Time PCR, Reverse Transcription

Specifications

Specifications

50 U/μL
Reverse Transcription Reagent
Separate components
Up to 5 kb
Reverse Transcription
MultiScribe
First-Strand cDNA
RT-PCR, RT-qPCR
Reverse Transcription
RNA
42°C
Yes
SDS
Product Certifications

For Research Use Only. Not for use in diagnostic procedures.