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Applied Biosystems™ Fast SYBR™ Green Master Mix DFS Item

Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.

Supplier:  Applied Biosystems™ 4385612

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Catalog No. 4385612



  • A fast, reliable, and cost-effective solution for real-time PCR applications without compromising sensitivity, specificity, dynamic range, or PCR efficiency
  • Real-time PCR results in as fast as 35 minutes
  • Capable of detecting very low copies of target
  • Minimizes primer-dimer and non-specific amplification
  • Consistent amplification across a wide dynamic range
  • Contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • Store at -5°C to -30°C

Includes the following components in an optimized buffer:

  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start
    • Minimizes non-specific product formation and enables reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision


Contains 1 × 5 mL vial. The 2X mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components. Sufficient for 500 reactions of 20 μL each.

Store at -5 to -30°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

SYBR Green
UP (Ultra Pure)
Dry Ice
Gene Expression
500 Reactions
5 mL
AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
ROX (Pre-mixed)
Real Time PCR SYBR Master Mix
1 x 5 mL
500 Reactions of 20 μL Each
DNA (Genomic), cDNA




For Research Use Only. Not for use in diagnostic procedures.