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Applied Biosystems™ Fast SYBR™ Green Master Mix DFS Item

Designed for speed, provides a fast, reliable and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range or PCR efficiency.

$136.00 - $5,140.00


Detection Method SYBR
Sample Type DNA (Genomic), cDNA
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
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Includes: SYBR Green I Dye, AmpliTaq Gold Fast DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference I and optimized buffer components

Requires: Template and primers

Products 6
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
4385610 View Documents Applied Biosystems™
1 x 1mL Each for $136.00
4385612 View Documents Applied Biosystems™
1 x 5mL Each for $582.00
4385616 View Documents Applied Biosystems™
2 x 5mL Each for $1,080.00
4385617 View Documents Applied Biosystems™
5 x 5mL Each for $2,595.00
4385618 View Documents Applied Biosystems™
10 x 5mL Each for $5,140.00
4385614 View Documents Applied Biosystems™
1 x 50mL Each for $4,790.00


  • Fast - Real-time PCR results in as fast as 35 minutes
  • Sensitive - Detect very low copies of target
  • Specific - Minimize primer-dimer and non-specific amplification
  • Reproducible - Consistent amplification across a wide dynamic range Fast SYBR™ Green Master Mix contains all of the components, excluding the template and primers, in a convenient 2X master mix
  • AmpliTaq™ Fast DNA Polymerase, UP, a highly purified DNA polymerase designed to allow instant hot start, minimizing non-specific product formation and enabling reactions to be set up at room temperature
  • SYBR™ Green I dye to enable detection of double-stranded DNA
  • Deoxynucleotides (dNTPs) to help maintain optimal PCR results
  • Uracil-DNA Glycosylase (UDG) designed to reduce carryover contamination
  • Passive internal reference based on proprietary ROX™ dye to enable increased precision

Order Info

Shipping Condition: Dry Ice



DNA (Genomic), cDNA
7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode

For Research Use Only. Not for use in diagnostic procedures.