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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1

Thermo Scientific™ GeneJET PCR Purification Kit

Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.

Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format Spin Column
Run Time 5 min.
High-throughput Compatibility Not High-throughput Compatible (Manual)
2

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Medium
Includes Label or Dye Yes
Overhang Blunt
Concentration 2X
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
Polymerase Phire Hot Start II DNA Polymerase
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Tissue
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
3

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

4

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
5

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

6

Invitrogen™ PureLink™ PCR Purification Kit  DFS Item

The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.

Shipping Condition Room Temperature
Content And Storage For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Format Kit
Sample Type PCR Products
Isolation Technology Silica Spin Column
Run Time 10 min.
8

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit  DFS Item
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT, TaqMan
Detection Method TaqMan
9

Invitrogen™ Precision gRNA Synthesis Kit  DFS Item

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

10

Applied Biosystems™ MagMAX™ Viral/Pathogen Binding Beads  DFS Item

These binding beads are included in the MagMAX Viral/Pathogen Nucleic Acid Isolation kits and MagMAX Microbiome Ultra Nucleic Acid Isolation Kit.

11

Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance High
Includes Label or Dye Yes
Overhang Blunt
Concentration 2X
For Use With (Application) Genotyping
PCR Method Direct PCR
Polymerase Phusion Blood II DNA Polymerase
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Blood
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
12

Invitrogen™ Cells-to-CT™ Stop Solution  DFS Item
GREENER_CHOICE

A component of the Cells-to-CT™ Kits.

13

Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)  DFS Item

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

14

Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit  DFS Item
GREENER_CHOICE

The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.

Shipping Condition Room Temperature
Target DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage

The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer.

Store at room temperature. Guaranteed stable for 6 months when properly stored.
Format Kit
Product Type PCR Clean-Up Kit
Sample Type PCR Products
Isolation Technology ChargeSwitch™ Chemistry
For Use With (Application) Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Line ChargeSwitch
15

MilliporeSigma™ Calbiochem™ EMPIGEN™ BB Detergent, 30% Solution

Can be used as detergent for solubilization of membrane components