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Invitrogen™ PureLink™ Pro 96 PCR Purification Kit

Catalog No. K310096A
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K310096A 4 plates kit
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Catalog No. K310096A Supplier Invitrogen™ Supplier No. K310096A
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Description

Includes

Four 96-well plates and sufficient reagents to perform four 96-well purifications

The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.

The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes. The PureLink™ Pro 96 PCR Purification Kits provide rapid and efficient removal of short primers, dNTPs, enzymes, short-failed PCR products, and salts from PCR products. The simple PureLink™ bind/wash/elute procedure facilitates routine purifications of double-stranded DNA fragments from 100bp to 12kb.

Improved plate design
The PureLink™ Pro 96 PCR Purification Kit provides optimized semi-skirted 96-well plates for improved performance and results. In addition, the plate nozzles with annular collar prevent cross-contamination of samples and improve drying of the silica membrane to prevent ethanol carryover. In addition, sample processing can be performed manually using a benchtop vacuum manifold, by centrifugation, or with an automated liquid handling platform. The semi-skirted plate design is compatible with most vacuum manifolds on robotic workstations.

PCR products purified using the PureLink™ Pro 96 PCR Purification Kit demonstrate outstanding performance in downstream applications such as cloning automated fluorescent DNA sequencing, PCR, restriction mapping, and labeling.

High Throughput-Compatible

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage The PureLink™ Pro 96 PCR Purification Kit includes 4 x 96-well plates, and sufficient reagents to perform 4 x 96-well purifications.

Store components at room temperature. All reagents are guaranteed stable for 12 months when properly stored.
Format Kit
Elution Volume Max 50 μL (centrifuge), 100 μL (vacuum), 150 μL
For Use With (Equipment) Biomek™ FX, Robotic Liquid Handling Systems, Vacuum Manifold, Eppendorf epMotion, Centrifuge, TECAN Freedom Evo, TECAN Genesis
High-throughput Compatibility High-throughput Compatible
Product Line PureLink
Product Type PCR Purification Kit
Purity or Quality Grade Molecular Biology Grade
Quantity 4 plates kit
Run Time 20 min.
Scale Mini
Shipping Condition Room Temperature
Target DNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application) Next-Generation Sequencing (NGS), Cloning
Final Product Type DNA
Isolation Technology Silica Plate
No. of Reactions 96 Preps
Sample Type PCR Products
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Frequently Asked Questions (FAQs)

If I do not use all the wells in the filter plate of the PureLink Pro 96 Purification System, can I use those wells at another time?

Yes. Keep the unused wells covered with Foil Tape. Please note that extra Foil Tape and Receiver Plates will be needed.
My centrifuge does not reach 3,000 x g. For the PureLink Pro 96 system, could I spin at a lower force for a longer time?

Generally speaking, yes. It should work the same if you can keep the same g (rcf) x time.

What is the minimum and maximum size DNA fragments that can be isolated using the PureLink Pro 96 PCR Purification kit?

The kit facilitates routine purifications of double-stranded DNA fragments from 100 bp to 12 kb.
What downstream applications can the purified PCR product from PureLink Pro 96 PCR Purificaiton kit be used for?

The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, cloning.

For Research Use Only. Not for use in diagnostic procedures.