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  7. Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Catalog No. F160S
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F160S 250 Reactions
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Catalog No. F160S Supplier Thermo Scientific™ Supplier No. F160S
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Description

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material like complex polysaccharides, polyphenols, and others.

Phire Plant Direct PCR Master Mix has been tested with leaves and seeds from a wide variety of plant species as well as fungi, bacteria, and yeast. The kit includes a complete set of optimized reagents and detailed protocols, making it an ideal choice for amplification of plant DNA.

Features

  • Direct PCR allows sample to be added directly to PCR reaction, reducing time-consuming and expensive DNA purification steps
  • Available Dilution & Storage protocol allows several PCR reactions from one sample
  • Specially engineered Phire Hot Start II DNA Polymerase for extremely short PCR protocol times
  • Master mix format with premixed gel loading dye minimizes risk of cross-contamination, reduces sample handling, and allows direct loading on gel

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

Notes

  • Annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

Concentration 2X
Content And Storage • 2X Phire Plant Direct PCR Master Mix, 2 x 1.25 mL
• Dilution Buffer, 1 x 5 mL
• 25 μM Control Primer Mix, 40 μL
• Nuclease-free water, 2 x 1.25 mL
• O'GeneRuler Express DNA Ladder, 50 μg (sufficient for 100 applications)

Store at -20°C.
GC-Rich PCR Performance Medium
PCR Method Direct PCR
Polymerase Phire Hot Start II DNA Polymerase
Reaction Speed Fast
Includes Label or Dye Yes
Product Type Direct PCR Master Mix
Quantity 250 reactions
Shipping Condition Wet or Dry Ice
For Use With (Application) Genotyping, Hot-start PCR
Hot Start Built-In Hot Start
No. of Reactions 250 Reactions
Overhang Blunt
Reaction Format SuperMix or Master Mix
Sample Type Plant
Starting Material DNA
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Frequently Asked Questions (FAQs)

Can I use frozen leaves with the Phire Plant Direct PCR Master Mix or Kit?

Yes, frozen (-20 degrees C and -70 degrees C), dried and fresh plant leaves can be used. We have succeeded in amplifying DNA directly from oak leaves stored at -20 degrees C for 10 years. However the highest amplification yields are achieved when using fresh samples.

For Research Use Only. Not for use in diagnostic procedures.