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Applied Biosystems™ MicroSEQ™ D2 rDNA Fungal PCR Kit
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Catalog No. 4349153
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Catalog No. 4349153 Supplier Applied Biosystems™ Supplier No. 4349153
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Description

This kit is the PCR component of the MicroSEQ™ Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi.

This kit is the PCR component of the MicroSEQ™ Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi. A protocol and Quick Reference Card are not included.

  • Sequence-based measurement ensures you get reproducible results in less than 24 hours.
  • Growth requirement-independent measurement enables you to identify fungi using objective criteria.
  • Universal domain-level primers eliminate up-front optimization.
  • MicroSEQ™ ID Analysis Software ensures accurate species identification.
  • Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
  • Requires minimal training; same work flow as the MicroSEQ™ Full Gene 16S rDNA Bacterial Identification Kit.

Specific and Easy to Use

Based on phylogenetic analysis of the D2 region of large-subunit ribosomal RNA gene sequences, this easy-to-use kit enables you to identify even the most difficult fungi, yeast, and oomycete plant pathogens. To ensure accurate and specific results, the kit's software compares your samples to a database of D2 sequences from over 1,000 validated type species.

The MicroSEQ™ ID System: A Complete, Integrated Solution

Streamlining every step in microbial identification, Applied Biosystems MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSEQ™ bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

Specifications

Content And Storage The kit contains reagents sufficient for 50 PCR reactions:
PCR Master Mix
Positive Control DNA
Negative Control (water)
After first use, store the kit in a PCR clean room at 2–8 °C
Detection Method Primer-probe
Technique PCR Amplification, SBT (Sequence-Based Typing)
For Use With (Equipment) Veriti Thermal Cycler, 9800 Fast Thermal Cycler
Libraries Fungal Library (> 1100 species), Customizable
Product Line MicroSEQ
Product Type PCR Kit
Quantity 1 Kit
Shipping Condition Dry Ice
Software MicroSEQ™ ID Analysis Software
Target D2 region of rRNA gene
Target Organism Class Fungi
For Use With (Application) Microbial Detection
No. of Reactions 50 Reactions (PCR)
Sample Type DNA (Genomic)
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For Research Use Only. Not for use in diagnostic procedures.