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Invitrogen™ Precision gRNA Synthesis Kit

Numéro de catalogue. A29377
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25 reactions
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Numéro de catalogue. Quantity
A29377 25 reactions
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For research use only. Not for use in diagnostic procedures.
Numéro de catalogue. A29377 Fournisseur Invitrogen™ Code fournisseur A29377
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For research use only. Not for use in diagnostic procedures.

Description

Comprend

BOX 1

  • 5X TranscriptAidTM Reaction Buffer
  • DNase I, RNase-free (1 U/μL)
  • Nuclease-free water
  • NTP mix (100 mM each of ATP, GTP, CTP,
  • UTP in Tris buffer)
  • Control gRNA forward and reverse primers (10 μM mix) 2

BOX 2

  • Binding Buffer
  • Wash Buffer 1 (concentrated)3
  • Wash Buffer 2 (concentrated)4
  • Nuclease-free water
  • Gene JETTM RNA Purification Micro Column & Collection Tubes
  • Elution Tubes, 1.5 mL

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short one-pot PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.

Features of the GeneArt Precision gRNA Synthesis Kit include:

  • Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
  • High yield (>10 ug) and concentration (>200 ng/uL) of gRNA

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Recommended Storage

gRNA Prep Kit, store at -5 to -30°C and gRNA Cleanup Kit, store at room temperature

Spécifications

Concentration 200 ng/μL
Content And Storage • gRNA Prep Kit, store at -5°C to -30°C
• - gRNA Cleanup Kit, store at room temperature (For better long-term performance, store purification columns at 2°C to 8°C)
Form Liquid
Format Kit
Reaction Speed Fast
Technique CRISPR-Cas9
Product Type gRNA Synthesis Kit
Promoter T7
Quantity 25 reactions
Shipping Condition Approved for shipment at Room Temperature or on Wet or Dry Ice
Sufficient For 25 Reactions
Yield >10 μg at >200 ng/μL of gRNA
Final Product Type RNA
No. of Reactions 25 Reactions
Starting Material Oligos (DNA)
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For Research Use Only. Not for use in diagnostic procedures.