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  7. Invitrogen™ PureLink™ PCR Micro Kit

Invitrogen™ PureLink™ PCR Micro Kit

Catalog No. K310250
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Catalog No. K310250 Supplier Invitrogen™ Supplier No. K310250
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Description

Includes

Columns, binding and wash buffers, recovery tubes, and the manual

PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.

PureLink™ PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.These highly purified DNA fragments and amplicons provide excellent results in downstream applications such as DNA sequencing, restriction digestion, cloning, PCR, and labeling. PureLink™ PCR Micro Kits provide a simple and rapid method for purifying and concentrating DNA fragments ranging in size from 100 bp-12 kb.

The unique micro-nozzle design allows up to 95% recovery of DNA fragments with as little as a 5μL elution volume. With less than a 10% coefficient of variance in the elution volume you can be sure that no ethanol is carried over from the wash steps giving you the best chance of success in downstream applications. Removal of primers, dNTPs, enzymes, and salts exceeds 98.5%. This kit can also efficiently remove unincorporated dye-labeled nucleotides from a cDNA labeling reaction.

Not High Throughput-Compatible (Manual)

Order Info

Shipping Condition: Room Temperature

Specifications

Content And Storage Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature.
Format Spin Column
Elution Volume Max 20 μL
Elution Volume Min 5 μL
High-throughput Compatibility Not High-throughput Compatible (Manual)
Product Line PureLink
Product Type PCR Micro Kit
Quantity 250 Preps
Run Time 10 min.
Separation Range 100 bp to 12 kb
Shipping Condition Room Temperature
Starting Material Volume 125 μL
Target DNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application) Cloning, Standard PCR
Isolation Technology Silica Spin Column
No. of Reactions 250 Preps
Sample Type PCR Products
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Frequently Asked Questions (FAQs)

What are the size limitations for the PureLink PCR Cleanup Micro Kit?

The size cut-off is 100 bp to 12 kb.
What are the minimum and maximum size ranges for DNA fragment cleanup using the PureLink PCR Clean-Up Kit?

The size range is between 100 bp and 12 kb. Our kit comes with two buffers, Binding Buffer (B2) and Binding Buffer HC (B3). The Binding Buffer HC (B3) eliminates primer-dimers and short failed PCR products that are smaller than 300 bp.

For Research Use Only. Not for use in diagnostic procedures.