
Gel Filtration Media and Columns
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Cytiva HiLoad™ 16/600 Superose™ 6 Prep Grade Preparative SEC Column
A prepacked SEC column for high-resolution purification of large biomolecules and protein complexes with sample volumes up to 5 mL
Cytiva Sephadex™ G50 Superfine Media
Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.
Plage de pH | 2 to 10 |
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Hauteur (impériale) | 3.93 in. |
Hauteur (métrique) | 10cm |
Format | Solid Bead |
Volume du lit | 9 to 11mg/g |
Type de produit | Sephadex G50 Media |
Diamètre (métrique) interne | 5cm |
Type d’échantillon | DNA |
Granulométrie | 100 to 300 μm in wet form, 50 to 150 μm in dry form |
Type de support | Cross-linked Dextran |
Plage de pH | 2 to 10 |
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Hauteur (impériale) | 3.93 in. |
Débit | < Darcy´s Law |
Type de produit | Sephadex G50 Media |
Diamètre (métrique) interne | 5cm |
Type d’échantillon | DNA |
Type de support | Cross-linked Dextran |
Contenu et stockage | 4 to 30°C |
À utiliser avec (application) | For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids |
Hauteur (métrique) | 10cm |
Format | Solid Bead |
Volume du lit | 9 to 11mg/g |
Granulométrie | 34 to 208 μm in wet form, 20 to 80 μm in dry form |
Pression max. | min 150 cm/h, bed height 10 cm, column 5 cm i.d. |
Cytiva HiTrap™ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.
Plage de pH | 3 to 12 |
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Hauteur (impériale) | 0.98 in. |
Hauteur (métrique) | 2.5cm |
À utiliser avec (équipement) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system |
Type de ligand | 10μM NHS groups/mL medium |
Matériau | Polypropylene |
Type de produit | NHS-Activated HP Column |
Granulométrie | 34 μm |
Pression max. | 42psi |
Type de support | 6% Highly Cross-linked Agarose |
Contenu et stockage | 4° to 8°C |
À utiliser avec (application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
Cytiva Sephacryl™ S-400 High Resolution Media
Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules
Cytiva Sephadex™ G-50 and G-100 DNA Grade Agarose Gels
Used for purification of DNA from small molecules. Cytiva illustra™ Sephadex™ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.
Plage de pH | 2 to 10 |
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Format | Beaded Gel |
À utiliser avec (équipement) | Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column |
Type de produit | G-50 and G-100 DNA Grade Agarose Gels |
Type d’échantillon | DNA |
Type de support | Cross-linked Dextran with Epichlorohydrin |
Contenu et stockage | 4° to 30°C |
Cytiva Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescience™ Superdex™ 200 Increase 5/150 GL Prepacked Tricorn™ Columns are designed for small scale preparative purification.
Cytiva Sephadex™ G15 Media
Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight
Cytiva HiTrap™ Capto™ Core 400
Designed for intermediate purification and polishing of viruses and other large biomolecules
Cytiva Sephadex G-50 Medium, 500g
Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.
Cytiva Sephadex™ G10 Media
Gel filtration medium for desalting and buffer exchange of peptides and small biomolecules
Plage de pH | 2 to 13 |
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Hauteur (impériale) | 11.81 in. |
Débit | < Darcy´s Law |
Type de produit | Sephadex G10 Media |
Diamètre (métrique) interne | 2.6cm |
Type d’échantillon | Peptides, Small Biomolecules |
Type de support | Cross-linked Dextran |
Contenu et stockage | 4 to 30°C |
À utiliser avec (application) | Desalting and Buffer exchange of Peptides and small biomolecules >700 M.W. |
Hauteur (métrique) | 30cm |
Format | Solid Bead |
Volume du lit | 2 to 3mL/g in Distilled Water |
Granulométrie | 55 to 165 μm in wet form, 40 to 120 μm in dry form |
Pression max. | 24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d |
Cytiva Sephadex™ G-100 Media
Commonly used for desalting and buffer exchange of very large biomolecules
Plage de pH | 2 to 10 |
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Format | Solid Bead |
Volume du lit | 15 to 20mL/g in Distilled Water |
Type de produit | Sephadex G-100 Media |
Type d’échantillon | Large biomolecules |
Quantité | 100 g |
Pression max. | 0.096 bar |
Type de support | Cross-linked Dextran |
Contenu et stockage | 4° to 30°C |
À utiliser avec (application) | Useful for desalting and Buffer exchange of very large biomolecules |
Cytiva Sepharose Agarose Gel Filtration Media
Choose from a wide range of bead-formed agarose-based gel filtration media. Cytiva Life Sciences™ Sepharose Agarose Gel Filtration Media shows good separation over a wide molecular weight range.
Format | Solid Bead |
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Type de produit | Sepharose Agarose Gel Filtration Media |
Quantité | 1 L |
À utiliser avec (application) | Allow characterization or cleaning up of samples containing components of diverse molecular weight |