
Gel Filtration Media and Columns
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Cytiva HiLoadâ„¢ 16/600 Superoseâ„¢ 6 Prep Grade Preparative SEC Column
A prepacked SEC column for high-resolution purification of large biomolecules and protein complexes with sample volumes up to 5 mL
Cytiva Sephadexâ„¢ G50 Superfine Media
Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadexâ„¢ G50 Superfine Media's small bead size gives higher efficiency.
Particle Size | 100 to 300 μm in wet form, 50 to 150 μm in dry form |
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Height (Metric) | 10cm |
Bed Volume | 9 to 11mg/g |
Format | Solid Bead |
pH Range | 2 to 10 |
Product Type | Sephadex G50 Media |
Sample Type | DNA |
Diameter (Metric) Inner | 5cm |
Support Type | Cross-linked Dextran |
Height (English) | 3.93 in. |
Cytiva Sephadexâ„¢ G-50 and G-100 DNA Grade Agarose Gels
Used for purification of DNA from small molecules. Cytiva illustraâ„¢ Sephadexâ„¢ G-50 and G-100 DNA Grade Agarose Gels purify DNA by gel filtration.
Content And Storage | 4° to 30°C |
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Format | Beaded Gel |
pH Range | 2 to 10 |
Product Type | G-50 and G-100 DNA Grade Agarose Gels |
For Use With (Equipment) | Auto Seq G-50 column, ProbeQuant G-50 micro column, MicroSpin G-50 column and NICK column |
Sample Type | DNA |
Support Type | Cross-linked Dextran with Epichlorohydrin |
Height (Metric) | 10cm |
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Content And Storage | 4 to 30°C |
Bed Volume | 9 to 11mg/g |
pH Range | 2 to 10 |
Diameter (Metric) Inner | 5cm |
For Use With (Application) | For the separation of molecules Mr >30 000 from molecules Mr <1500 such as labeled protein or DNA from unconjugated dyesFor removeing small nucleotides from longer chain nucleic acids |
Max. Pressure | min 150 cm/h, bed height 10 cm, column 5 cm i.d. |
Particle Size | 34 to 208 μm in wet form, 20 to 80 μm in dry form |
Flow Rate | < Darcy´s Law |
Format | Solid Bead |
Product Type | Sephadex G50 Media |
Sample Type | DNA |
Support Type | Cross-linked Dextran |
Height (English) | 3.93 in. |
Cytiva Sephacrylâ„¢ S-400 High Resolution Media
Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules
Cytiva HiTrapâ„¢ NHS-Activated HP Columns
Couple ligands containing primary amines easily. Cytiva Life Sciencesâ„¢ HiTrapâ„¢ NHS-Activated HP Columns are prepacked with NHS activated Sepharoseâ„¢ High Performance.
Particle Size | 34 μm |
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Ligand Type | 10μM NHS groups/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 8°C |
pH Range | 3 to 12 |
Product Type | NHS-Activated HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTAâ„¢ design or FPLC system |
Material | Polypropylene |
For Use With (Application) | Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva Superdexâ„¢ 200 Increase 5/150 GL Prepacked Tricornâ„¢ Columns
For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000. Cytiva Lifescienceâ„¢ Superdexâ„¢ 200 Increase 5/150 GL Prepacked Tricornâ„¢ Columns are designed for small scale preparative purification.
Cytiva Sephadexâ„¢ G15 Media
Gel filtration medium for desalting and buffer exchange for small biomolecules greater than 1500 molecular weight
Cytiva HiTrapâ„¢ Captoâ„¢ Core 400
Designed for intermediate purification and polishing of viruses and other large biomolecules
Cytiva Sephadex G-50 Medium, 500g
Suitable for large scale separation. Cytiva Sephadex G-50 Medium, 500g is a well-established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight.
Cytiva HiLoadâ„¢ Superdexâ„¢ 200 PG XK Column
Prepacked XK columns designed for high-resolution preparative gel filtration chromatography.
Content And Storage | 4 to 30°C, 20% Ethanol |
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Cytiva HiTrapâ„¢ Heparin HP Columns
Purify DNA-binding proteins, coagulation factors, lipoproteins and protein synthesis factors. Cytiva Life Sciencesâ„¢ HiTrapâ„¢ Heparin HP Columns are prepacked with Heparin Sepharoseâ„¢ High Performance for high-resolution purification.
Particle Size | 34 μm |
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Ligand Type | 10mg Heparin/mL medium |
Height (Metric) | 2.5cm |
Content And Storage | 4° to 30°C |
pH Range | 5 to 10 |
Product Type | Heparin HP Column |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTAâ„¢ design or FPLC system |
Material | Polypropylene |
For Use With (Application) | Purification of Antithrombin III, coagulation facto rs and other Plasma Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva HiTrapâ„¢ Blue HP Columns
For purifying albumin, enzymes including NAD+ and NADP+, coagulation factors, interferons and related proteins
Particle Size | 34 μm |
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Height (Metric) | 2.5cm |
Content And Storage | 4° to 30°C |
pH Range | 4 to 12 |
Product Type | Blue HP Columns |
For Use With (Equipment) | Syringe, peristaltic pump or liquid chromato graphy system such as AKTAâ„¢ design or FPLC system |
Sample Type | Albumin, Interferon, Nucleotide-dependent Enzymes, a2-macroglobulin, Coagulation Factors and Related Proteins |
Material | Polypropylene |
For Use With (Application) | Purifying wide range of biomolecules, including albumin, interferon, a broad range of nucleotide-dependent Enzymes, alpha2-macroglobulin, coagulation facto rs and related Proteins |
Support Type | 6% Highly Cross-linked Agarose |
Max. Pressure | 42psi |
Height (English) | 0.98 in. |
Cytiva Superoseâ„¢ Media Prep Grade Lab Packs
High performance gel filtration media for scaleup to preparative purifications of biomolecules
Particle Size | 30 to 40 μm |
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Flow Rate | 40 cm/hr. |
Bed Volume | ≈100 mL |
pH Range | 3 to 12 |
For Use With (Equipment) | AKTAâ„¢ design pump or system |
Sample Type | Proteins, Peptides, Polynucleotides, Wide Variety of Biomolecules |
Quantity | Each |
For Use With (Application) | Separations of Proteins and other biomolecules according to size |
Support Type | Highly Cross-linked Agarose |