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ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

3

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

4

Thermo Scientific™ Stop Solution for TMB Substrates

Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).

5

Invitrogen™ Luminescence Enhancer For Solution Assays, Emerald-II™ Enhancer  DFS Item

The Emerald-II chemiluminescence enhancer significantly reduces aqueous quenching and tunes the wavelength of light emission.

6

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

7

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

8

Invitrogen™ Galacton-Star™ Substrate (50X Concentrate)  DFS Item

Concentrated chemiluminescent substrate for E.coli β-galactosidase assays. Thermo Scientific Galacton-Star Substrate (50X Concentrate) is highly sensitive which makes it ideal for detection of weak expression and for transfection normalization with o

Shipping Condition Room Temperature
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Beta-Gal
Detection Method Chemiluminescence
9

Invitrogen™ cAMP-Screen™ Assay/Lysis Buffer  DFS Item

For use with the cAMP-Screen or cAmp-Screen Direct™ Immunoassay System.

10

Invitrogen™ Galacto-Light™ Reaction Buffer Diluent with Galacton-Plus™ Substrate  DFS Item

Established system with extended light emission and high sensitivity for reporter gene assays. Thermo Scientific Galacto-Light Reaction Buffer Diluent and the Galacton-Plus chemiluminescent substrate solution are used with the Galacto-Light Plus Reporter

11

Invitrogen™ cAMP-Screen™ Wash Buffer  DFS Item

For use with the cAMP-Screen or cAMP-Screen Direct™ Immunoassay system.

12

Thermo Scientific™ ELISA Blocker Blocking Buffer

Increases signal-to-noise ratio and minimizes background

13

Thermo Scientific™ ELISA Wash Buffer (30X)

Concentrated wash buffer for efficient use of storage in the lab

14

Molecular Probes™ CDP-Star™ Substrate (0.25 mM Ready-To-Use)  DFS Item

The CDP-Star™ Substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Product Line CDP-Star, NovaBright
15

Invitrogen™ ELISA-Light™ Immunoassay System with CDP-Star™ and Sapphire-II™ Substrate/Enhancer Solution  DFS Item

Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.